Anti-NFL (Neurofilament Light Chain) Antibody

Name: Anti-NFL (Neurofilament Light Chain) Antibody
Brand: Aves Labs
SKU: NFL
Price: 470.0 USD
Availability: InStock

4 Citations

Three antipeptide antibodies were generated in chickens against sequences shared between the mouse (P08551) and human (P07196) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Immunohistochemical staining of a paraffin-embedded section through mouse cerebellum. Anti-NFL immunoreactivity (Aves Labs, 1:500 dilution) was detected using HRP-labeled goat anti-chicken IgY (Aves Cat.No. H-1004).

Immunocytochemical staining of mouse brain cortical neurons in culture. NFL immunoreactivity is red and yellow. Blue staining is DAPI nuclear staining. Anti-NFL (1:1000 dilution). Dr. Felix Eckenstein, University of Vermont.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: NFL

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neurofilament Light Chain (NFL)

Target Description

Human Neurofilament Light Chain (NFL) is a 61,516 dalton protein (543 amino acids) expressed in the axons of CNS and PNS neurons, where it plays a role in maintaining the structural integrity of the axon. Mutations of the NFL gene product are responsible for some forms of autosomal dominant Charcot-Marie-Tooth neuropathies in humans (i.e., CMT type 1F).

Format

Affinity-Purified IgY

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFL NF68 NFL

Molecular Weight

70 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313553

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NF-L gene product, but are shared between the human (P07196, NCBI) and mouse (P08551, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Neurofilament light polypeptide (NF-L) (68 kDa neurofilament protein) (Neurofilament triplet L protein)

UniProt ID

P07196

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
38883841	not listed	Kumar, A, et al. 2024. MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A. iScience, 109994.
29404402	1:500	Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, .

Immunocytochemistry: Rat
PMID	Dilution	Publication
30038033	1:500	Lee, S.J., et al. 2018. hnRNPs interacting with mRNA localization motifs define axonal RNA regulons. Molecular & Cellular Proteomics, 2091.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28828403	1:1,000	Lerch, J.K., et al. 2018. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs. eNeuro, .

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Anti-NFL (Neurofilament Light Chain) Antibody

SKU: NFL

Product Details

Product Specific References for Applications and Species

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