NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.
Primary Antibody
Human, Mouse, Rat
ICC, IHC, WB
Chicken
IgY Fraction
NEFH KIAA0845 NFH
200-220 kDa
Product Specific References for Applications and Species
Immunocytochemistry: Mouse | ||
PMID | Dilution | Publication |
35821510 | 1:2000 | Rogers, E.A., et al. 2022. Utilizing novel TBI-on-a-chip device to link physical impacts to neurodegeneration and decipher primary and secondary injury mechanisms. Scientific reports, 11838. |
29404402 | 1:500 | Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, . |
28828403 | 1:1000 | Lerch, J.K., et al. 2018. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs. eNeuro, . |
Immunocytochemistry: Rat | ||
PMID | Dilution | Publication |
30038033 | 1:500 | Lee, S.J., et al. 2018. hnRNPs interacting with mRNA localization motifs define axonal RNA regulons. Molecular & Cellular Proteomics, 2091-2106. |
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
37607547 | 1:500 | Koutsioumpa, C, et al. 2023. Skin-type-dependent development of murine mechanosensory neurons. Developmental Cell, . |
37541195 | 1:1000 | Wolfson, RL, et al. 2023. DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon. Cell, 3368-3385.e18. |
37416774 | not listed | Hu, M, et al. 2023. Defective Neurite Elongation and Branching in Nibp/Trappc9 Deficient Zebrafish and Mice. International Journal of Biological Sciences, 3226-3248. |
37350129 | 1:1500 | Glaser, EP, et al. 2023. Effects of Acute Ethanol Intoxication on Spinal Cord Injury Outcomes in Female Mice. Journal of Neurotrauma, 0. |
37208933 | 1:500 | Pukos, N, et al. 2023. Chronic Demyelination and Myelin Repair After Spinal Cord Injury in Mice: A Potential Link for Glutamatergic Axon Activity. Glia, 2093-2116. |
36788334 | 1:1000 | Lopez, J.A., et al. 2023. Caldendrin Represses Neurite Regeneration and Growth in Dorsal Root Ganglion Neurons. Scientific Reports, 2608. |
36745682 | 1:5000 | Tang, A.T., et al. 2023. Cell-Autonomous Requirement for ACE2 Across Organs in Lethal Mouse SARS-CoV-2 Infection. PLoS Biology, e3001989. |
36370710 | 1:5000 | Wang, X., et al. 2022. Driving Axon Regeneration By Orchestrating Neuronal And Non-Neuronal Innate Immune Responses Via The IFNγ-cGAS-STING Axis. Neuron, . |
36284453 | not listed | Cantu-Guerra, H.L., et al. 2022. Cochlear Hair Cell Innervation is Dependent on a Modulatory Function of Semaphorin-3A. Developmental Dynamics, 124-144. |
36087584 | 1:300 | Orr, B., et al. 2022. Activation and expansion of presynaptic signaling foci drives presynaptic homeostatic plasticity. Neuron, 3743-3759. |
35568187 | not listed | Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114. |
35552394 | 1:200 | Wang, Y., et al. 2022. The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development. Development, . |
35373589 | 1:1500 | Stewart, A., et al. 2022. Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury. Journal of neurotrauma, 1075-1089. |
34789880 | 1:500 | Emanuel, A.J., et al. 2021. Cortical responses to touch reflect subcortical integration of LTMR signals. Nature, 680-701. |
34731626 | 1:5000 | Parpaite, T., et al. 2021. Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions. Cell reports, 109914. |
34730998 | 1:1000 | Xu, Q., et al. 2021. Astrocytes contribute to pain gating in the spinal cord. Science advances, 6287. |
34637701 | 1:500 | Lehnert, B.P., et al. 2021. Mechanoreceptor synapses in the brainstem shape the central representation of touch. Cell, 5608-5621. |
33422552 | 1:2500 | Stewartac, A.N., et al. 2021. Mitochondria exert age-divergent effects on recovery from spinal cord injury. Experimental Neurology, 113597. |
33422552 | 1:2500 | Stewart, A.N., et al. 2021. Mitochondria exert age-divergent effects on recovery from spinal cord injury. Experimental Neurology, 113597. |
33333021 | 1:500 | Huang, S., et al. 2020. Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential. Cell, 441-459. |
32675174 | 1:1000 | Wang, Z., et al. 2020. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. eNeuro, . |
32554568 | 1:500 | Neubarth, N.L., et al. 2020. Meissner Corpuscles and Their Spatially Intermingled Afferents Underlie Gentle Touch Perception. Science, eabb2751. |
31862897 | 1:200 | Morrey, J.D., et al. 2019. Zika virus infection causes temporary paralysis in adult mice with motor neuron synaptic retraction and evidence for proximal peripheral neuropathy. Scientific Reports, 19531. |
31309927 | 1:400 | Stavoe, A.K.H, et al. 2019. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.. Elife, e44219. |
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31209173 | 1:2000 | Jung, J.S., et al. 2019. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development.. Journal of Neuroscience, 6425-6438. |
30866042 | 1:400 | Goel, M., et al. 2019. Differential expression and sub-cellular localization of Copines in mouse retina. Journal of Comparative Neurology, 2245-2262. |
30726728 | 1:500-1:1000 | Zhang, M., et al. 2019. Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice. Cell Reports, 1419-1431. |
30686732 | 1:500 | Green, D.P., et al. 2019. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron, 412-420. |
30478340 | 1:10000 | Colina, C., et al. 2018. Selective tracking of FFAR3-expressing neurons supports receptor coupling to N-type calcium channels in mouse sympathetic neurons. Scientific reports, 17379. |
30453022 | 1:500 | Zhang, B., et al. 2018. Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury. Brain, Behavior, and Immunity, 139-150. |
30406183 | 1:100 | Micheva, K.D. , et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, . |
29874580 | 1:1000 | Kridsada, K., et al. 2018. Roof Plate-Derived Dorsal Midline Radial Glial-like Cells Promote Longitudinal Ascending Axon Growth of Rapidly Adapting Mechanoreceptors during Development. Cell Reports, 2928-2941. |
29536590 | 1:5000 | Harley, R.J., et al. 2018. Neuronal Cell Adhesion Molecule (NrCAM) is Expressed by Sensory Cells in the Cochlea and is Necessary for Proper Cochlear Innervation and Sensory Domain Patterning During Development. Developmental Dynamics, 934-950. |
29476408 | 1:200 | Zukor, K., et al. 2018. Zika virus-induced acute myelitis and motor deficits in adult interferon αβ/γ receptor knockout mice. Journal of NeuroVirology, 273-290. |
29329211 | 1:200 | Morrey, J.D., et al. 2018. Causation of Acute Flaccid Paralysis by Myelitis and Myositis in Enterovirus-D68 Infected Mice Deficient in Interferon αβ/γ Receptor Deficient Mice. Viruses, 33. |
29279310 | 1:1000 | Hesp, Z.C., et al. 2018. Proliferating NG2-Cell-Dependent Angiogenesis and Scar Formation Alter Axon Growth and Functional Recovery After Spinal Cord Injury in Mice. The Journal of Neuroscience, 1366-1382. |
28817806 | not listed | Ghitani, N., et al. 2017. Specialized Mechanosensory Nociceptors Mediating Rapid Responses to Hair Pull. Neuron, 944-954. |
28647856 | 1:500 | Shih, Y., et al. 2017. Glial and Neuronal Protein Tyrosine Phosphatase Alpha (PTPα) Regulate Oligodendrocyte Differentiation and Myelination. Journal Molecular Neuroscience, 329-343. |
28381129 | not listed | Orr, M.B., et al. 2017. Compression Decreases Anatomical and Functional Recovery and Alters Inflammation after Contusive Spinal Cord Injury. Journal of Neurotrauma, 2342-2352. |
28264978 | 1:200 | Freria, C.M., et al. 2017. Deletion of the fractalkine receptor, CX3CR1, improves endogenous repair, axon sprouting and synaptogenesis after spinal cord injury in mice. The Journal of Neuroscience, 3568-3587. |
28162807 | 1:1000 | François, A., et al. 2017. A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins. Neuron, 822-839. |
28041852 | 1:1000 | Abraira, V.E., et al. 2017. The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell, 295-310. |
28168703 | 1:1000 | Geary, M.B., et al. 2016. Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle & Nerve, 143-151. |
28002412 | 1:1000 | Nonomura, K., et al. 2016. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature, 176-181. |
27707970 | 1:500 | Fleming, M.S., et al. 2016. A RET-ER81-NRG1 Signaling Pathway Drives the Development of Pacinian Corpuscles. The Journal of Neuroscience, 10337-10355. |
27596335 | 1:500 | Zhang, B., et al. 2016. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiology of Aging, 157-167. |
26356988 | 1:400 | Sajgo, S., et al. 2015. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. Journal of Comparative Neurology, 1033-1061. |
25451251 | 1:5000 | Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346. |
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
36759186 | 1:200 | Arbat-Plana, A., et al. 2023. Massive Loss of Proprioceptive Ia Synapses in Rat Spinal Motoneurons after Nerve Crush Injuries in the Postnatal Period. eNeuro, . |
33468672 | 1:200 | Meregalli, C., et al. 2021. Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy. PNAS, . |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
37416774 | not listed | Hu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248. |