Anti-Neuropilin-1 (a1 CUB Domain) Antibody

Name: Anti-Neuropilin-1 (a1 CUB Domain) Antibody
Brand: PhosphoSolutions
SKU: NP2111
Price: 340.0 USD
Availability: InStock

2 Citations

Our Neuropilin-1 (a1 CUB Domain) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Neuropilin-1 (a1 CUB Domain) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).

Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NP2111) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide (NX2115) in the presence of the anti-NRP-1 antibody (C,F).

Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with anti-HA (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Go to Full Product Details

SKU: NP2111

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neuropilin-1 (a1 CUB Domain)

Scientific Background

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

NRP1

Molecular Weight

130

Immunogen

Neuropilin-1 synthetic peptide (coupled to carrier protein) corresponding to amino acids within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of COS-7 cells transfected with mouse neuropilin-1. In immunocytochemistry, this antibody co-localizes with anti-HA in Cos-7 cells transfected with HA-tagged mouse neuropilin-1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

NRP1, VEGF 165, VEGFR

UniProt ID

O14786

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
28507510	1:250	Sano, N., et al. 2017. Enhanced Axonal Extension of Subcortical Projection Neurons Isolated from Murine Embryonic Cortex using Neuropilin-1. Frontiers in Cellular Neuroscience, 123.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32140099	1:500	Samata, B., et al. 2020. L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain. Frontiers in Cellular Neuroscience, 31.
28507510	1:250	Sano, N., et al. 2017. Enhanced Axonal Extension of Subcortical Projection Neurons Isolated from Murine Embryonic Cortex using Neuropilin-1. Frontiers in Cellular Neuroscience, 123.

Recover password

Create my account

Anti-Neuropilin-1 (a1 CUB Domain) Antibody

SKU: NP2111

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Neuropilin-1 (a1 CUB Domain) Antibody

Related Products

Recently Viewed