Anti-Neuron-Specific Enolase Antibody

Name: Anti-Neuron-Specific Enolase Antibody
Brand: Aves Labs
SKU: NSE
Price: 470.0 USD
Availability: InStock

2 Citations

Two antipeptide antibodies were generated in chickens against a sequence shared between the rat (AAA41119) and human (NP_001966) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Western blot of adult mouse brain homogenate showing a single band at the correct MW (47 kDa), dilution 1:100000.

Immunocytochemical staining of NSE (green, 1:1000 dilution) and Glial Fibrillary Acidic Protein (GFAP, rabbit, 1:1000 dilution). Photomicrograph by Dr. Gerry Shaw (EnCor Biotechnology).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: NSE

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neuron-Specific Enolase (NSE) Type 2

Target Description

Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate and is an essential enzyme in energy metabolism in nervous tissues.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

ENO2

Molecular Weight

47 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313555

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:10000-1:20000
ICC: 1:10000-1:20000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Gamma-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 2) (Neural enolase) (Neuron-specific enolase) (NSE)

UniProt ID

P09104

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
27339393	1:500	Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice. Neuropsychopharmacology, 2920-2931.

Western Blot: Rat
PMID	Dilution	Publication
20698760	1:500	Ottens, A.K., et al. 2010. Neuroproteomics: a biochemical means to discriminate the extent and modality of brain injury. Journal of Neurotrauma, 1837-1852.

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Anti-Neuron-Specific Enolase Antibody

SKU: NSE

Product Details

Product Specific References for Applications and Species

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