Anti-Netrin-1 Antibody
An antipeptide antibody was generated in chickens against a sequence shared between the mouse (O09118) and human (O95631) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.
Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. No. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (Aves Cat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.
Product Specific References for Applications and Species
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 23161783 | 1ug/ml | Goldberg, D., et al. 2013. Slit/Robo‐mediated chemorepulsion of vagal sensory axons in the fetal gut. Developmental Dynamics, 45184. |
| 16917820 | 1ug/ml | Ratcliffe, E.M., et al. 2006. Netrin/DCC‐mediated attraction of vagal sensory axons to the fetal mouse gut. Journal of Comparative Neurology, 567-580. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 30576266 | not listed | Ko, S.Y., et al. 2019. Netrin-1-like-immunoreactivity Coexpresses With DCC and Has a Differential Level in the Myenteric Cholinergic and Nitrergic Neurons of the Adult Mouse Colon. Journal of Histochemistry and Cytochemistry, 335-349. |
| 21485011 | 1ug/ml | Ratcliffe, E.M., et al. 2011. Enteric neurons synthesize netrins and are essential for the development of the vagal sensory innervation of the fetal gut. Developmental Neurobiology, 362-373. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 21485011 | 1ug/ml | Ratcliffe, E.M., et al. 2011. Enteric neurons synthesize netrins and are essential for the development of the vagal sensory innervation of the fetal gut. Developmental Neurobiology, 362-373. |