Anti-Netrin-1 Antibody

Name: Anti-Netrin-1 Antibody
Brand: Aves Labs
SKU: NET
Price: 470.0 USD
Availability: InStock

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (O09118) and human (O95631) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.

Neurosphere (organoid) cultures of e13 mouse brain were cultured for 2 weeks, and then paraformaldehyde (2%) fixed. After extensive washing, the cultures were incubated with Netrin-1 antibody (Cat. NET; 1:500 dilution), washed, and then treated with fluorescein-labeled goat anti-chicken IgY (AvesCat. No. F-1005; 1:500 dilution). Hoda Ilias, Aves Labs.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

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Data Sheet

SKU: NET

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Netrin-1

Scientific Background

Murine netrin-1 precursor is a 67,768 dalton protein (604 amino acids) expressed by subpopulations of cells within the developing CNS, as well as in various non-neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of "commissural neurons" from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

NTN1 NTN1L

Molecular Weight

67 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313551

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the netrin-1 gene product, but was shared between the human (O_095631, NCBI) and mouse (O_09118, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: Not Recommended
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Netrin-1 (Epididymis tissue protein Li 131P)

UniProt ID

O95631

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
23161783	1ug/ml	Goldberg, D., et al. 2013. Slit/Robo‐mediated chemorepulsion of vagal sensory axons in the fetal gut. Developmental Dynamics, 45184.
16917820	1ug/ml	Ratcliffe, E.M., et al. 2006. Netrin/DCC‐mediated attraction of vagal sensory axons to the fetal mouse gut. Journal of Comparative Neurology, 567-580.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
30576266	not listed	Ko, S.Y., et al. 2019. Netrin-1-like-immunoreactivity Coexpresses With DCC and Has a Differential Level in the Myenteric Cholinergic and Nitrergic Neurons of the Adult Mouse Colon. Journal of Histochemistry and Cytochemistry, 335-349.
21485011	1ug/ml	Ratcliffe, E.M., et al. 2011. Enteric neurons synthesize netrins and are essential for the development of the vagal sensory innervation of the fetal gut. Developmental Neurobiology, 362-373.

Immunohistochemistry: Rat
PMID	Dilution	Publication
21485011	1ug/ml	Ratcliffe, E.M., et al. 2011. Enteric neurons synthesize netrins and are essential for the development of the vagal sensory innervation of the fetal gut. Developmental Neurobiology, 362-373.

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Anti-Netrin-1 Antibody

SKU: NET

Product Details

Product Specific References for Applications and Species

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