Anti-N-Cadherin (C-terminal region) Antibody

Name: Anti-N-Cadherin (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CP1751
Price: 285.0 USD
Availability: InStock

4 Citations

Our N-Cadherin (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat N-Cadherin (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IP.

Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.

Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

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Data Sheet

SKU: CP1751

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

N-Cadherin (C-terminal region)

Scientific Background

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

CDH2

Molecular Weight

130

Immunogen

unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids from the C-terminal region of human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200
IP: 1:100

Conjugate

Unconjugated

Specificity

The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Cadherin-2, Neural-Cadherin, CD325

UniProt ID

P19022

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Rat

Western Blot: Human
PMID	Dilution	Publication
22825629	1:10,000	Wu, Y., et al. 2012. The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer. International Journal of Oncology, 1337-1346.
22735690	not listed	Sato, F., et al. 2012. The basic helix-loop-helix transcription factor DEC2 inhibits TGF-β-induced tumor progression in human pancreatic cancer BxPC-3 cells. International Journal of Molecular Medicine, 495-501.
18979298	not listed	Ferreri, D.M., et al. 2008. N-cadherin levels in endothelial cells are regulated by monolayer maturity and p120 availability. Cell Communication and Adhesion, 333-349.

Western Blot: Rat
PMID	Dilution	Publication
24396070	not listed	Chang, Y.J., et al. 2014. SH2B1β interacts with STAT3 and enhances fibroblast growth factor 1-induced gene expression during neuronal differentiation. Molecular and Cellular Biology, 1003-1019.

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Anti-N-Cadherin (C-terminal region) Antibody

SKU: CP1751

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-N-Cadherin (C-terminal region) Antibody

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