Anti-MMACHC Antibody (N230/21)
Our Anti-MMACHC mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N230/21. It detects human and mouse MMACHC, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Product Specific References for Applications and Species
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 27771510 | 1:1000 | Bassila, C., et al. 2016. Methionine synthase and methionine synthase reductase interact with MMACHC and with MMADHC. Biochimica et Biophysica Acta. Molecular Basis of Disease, 103-112. |
| 23270877 | not listed | Mah, W., et al. 2013. Subcellular location of MMACHC and MMADHC, two human proteins central to intracellular vitamin B(12) metabolism.. Molecular Genetics and Metabolism, 112-118. |
| 22832074 | 1:3000 | Deme, J.C., et al. 2012. Structural features of recombinant MMADHC isoforms and their interactions with MMACHC, proteins of mammalian vitamin B12 metabolism.. Molecular Genetics and Metabolism, 352-362. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35013307 | 1:1000 | Chern, T., et al. 2022. Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. Nature Communications, 134. |
| 32941884 | 1:1000 | Chern, T., et al. 2020. Mouse models to study the pathophysiology of combined methylmalonic acidemia and homocystinuria, cblC type. Developmental Biology, 1-13. |
| 24889031 | 1:2500 | Moreno-Garcia, M.A., et al. 2014. The Mmachc gene is required for pre-implantation embryogenesis in the mouse.. Molecular Genetics and Metabolism, 198-204. |