Our Anti-Kv2.1 K+ channel (external) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K39/25. It detects human, mouse, and rat Kv2.1 K+ channel (external), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Surface immunofluorescence (no detergent permeabilization) of cultured rat hippocampal neuron transfected with HA-tagged Kv2.1 plasmid. Red = K39/25. Scale bar = 20 m. Reproduced from 2000 Lim et al Neuron (PMID 10719893).
Potassium voltage-gated channel subfamily B member 1 is encoded by the gene KCNB1. KCNB1 is a member of the potassium channel family, B (Shab) (TC 1.A.1.2) subfamily, Kv2.1/KCNB1 sub-subfamily. KCNB1 is a delayed rectifier potassium channel that mediates transmembrane potassium transport. KCNB1 contributes to the regulation of action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells, and endocrine cells, and plays a role in homeostatic attenuation of electrical excitability throughout the brain. KCNB1 is expressed in the brain, heart, lung, liver, colon, kidney, and adrenal gland. Diseases associated with KCNB1 include early infantile epileptic encephalopathy 26, undetermined early-onset epileptic encephalopathy, visual epilepsy, and alacrima, achalasia, and mental retardation syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K39/25
IgG2a
ICC, IHC, WB
Mouse
KCNB1
105-125 kDa (varies with cell background due to phosphorylation)
Synthetic peptide amino acids 211-229 (LPELQSLDEFGQSTDNPQL, extracellular S1-S2 loop) of rat Kv2.1 (accession number NP_037318)
Human, Mouse, Rat
AB_2131649
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.85
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Potassium voltage-gated channel subfamily B member 1 (Delayed rectifier potassium channel 1) (DRK1) (h-DRK1) (Voltage-gated potassium channel subunit Kv2.1)
David, J.P., et al. 2019. Deletion in mice of X-linked, Brugada syndrome- and atrial fibrillation-associated Kcne5 augments ventricular KV currents and predisposes to ventricular arrhythmia. FESEB Journal, 2537-2552.
Bocksteins, E., et al. 2009. Conserved negative charges in the N-terminal tetramerization domain mediate efficient assembly of Kv2.1 and Kv2.1/Kv6.4 channels.. The Journal of Biological Chemistry, 31625-31634.
Mohapatra, D.P., et al. 2008. Interdomain cytoplasmic interactions govern the intracellular trafficking, gating, and modulation of the Kv2.1 channel.. Journal of Neuroscience, 4982-4994.
Nieves-Cintrón, M., et al. 2015. Selective down-regulation of KV2.1 function contributes to enhanced arterial tone during diabetes.. The Journal of Biological Chemistry, 7918-7929.
Kirizs, T., et al. 2014. Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.. European Journal of Neuroscience, 1771-1783.
Nataraj, K., et al. 2010. Visual deprivation suppresses L5 pyramidal neuron excitability by preventing the induction of intrinsic plasticity.. Neuron, 750-762.
Battonyai, I., et al. 2014. Potassium channels in the central nervous system of the snail, Helix pomatia: localization and functional characterization.. Neuroscience, 87-101.
Jensen, C.S., et al. 2014. Specific sorting and post-Golgi trafficking of dendritic potassium channels in living neurons.. The Journal of Biological Chemistry, 10566-10581.
Zhao, X., et al. 2014. Amigo adhesion protein regulates development of neural circuits in zebrafish brain.. The Journal of Biological Chemistry, 19958-19975.
Hawkins, N.A., et al. 2021. Epilepsy and neurobehavioral abnormalities in mice with a dominant-negative KCNB1 pathogenic variant. Neurobiology Disorders, 105141.
Frazzini, V., et al. 2016. Altered Kv2.1 functioning promotes increased excitability in hippocampal neurons of an Alzheimer''s disease mouse model.. Cell Death & Disease, e2100.
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