Anti-GluR1-Subunit (Ser831) Antibody
Our Anti-GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Mouse, Rat
Canine, Chicken
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
GRIA1
100 kDa
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 22427672 | not listed | Jakan-Sakrikar, N., et al. 2012. Substrate-selective and calcium-independent activation of CaMKII by α-actinin. Journal of Biological Chemistry, 287(19), pp.15275-15283. |
| 21610080 | not listed | Jiao, Y., et al. 2011. Characterization of a Central Ca2+/Calmodulin-dependent Protein Kinase IIα/β Binding Domain in Densin That Selectively Modulates Glutamate Receptor Subunit Phosphorylation. Journal of Biological Chemistry 286: 24806 - 24818. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37648853 | 1:2000 | Tullis, JE, et al. 2023. LTP induction by structural rather than enzymatic functions of CaMKII. Nature, 146-153. |
| 33853773 | 1:1000 | Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300. |
| 26255006 | 1:500 | Pasek, J.G., et al. 2015. Differential CaMKII regulation by voltage-gated calcium channels in the striatum. Molecular and Cellular Neuroscience, 68, 234-243. |
| 24907515 | not listed | Mao, L.M., et al. 2014. Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European Journal of Pharmacology, 738, 237-244. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. Journal Neuroscience, 30: 8263-8273. |
| 17923095 | not listed | Hu,H., et al. 2007. Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31994358 | 1:500 | Mao, L.M., et al. 2018. Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain. Brain and Behavior, p.e01543. |
| 29432883 | not listed | Mao, L.M., et al. 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93. |
| 29242119 | not listed | Xue, B., et al. 2017. Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European Journal of Pharmacology. 2017 Dec 11;820:45-52. |
| 28578480 | not listed | Lameth, J., et al. 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16. |
| 27060412 | not listed | Xue, B., et al. 2017. Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65. |
| 26706598 | not listed | O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of Disease, 87, 134-144. |
| 26658861 | 1:1000 | Bean, L.A., et al. 2015. Re-opening the critical window for estrogen therapy. Journal of Neuroscience, 35(49), pp.16077-16093. |
| 25689263 | 1:1000 | Mao, L.M., et al. 2015. Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of Neurochemistry. Jun;133(6):795-805. |
| 24780380 | 1:500 | Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12. |
| 24231469 | 1:1000 | Xue, B., et al. 2014. Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry International, 64, 48-54. |
| 24056996 | 1:1000 | Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Molecular Pharmacology, 84(6), 834-843. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263 - 8273. |
| 20353941 | not listed | Coultrap, S.J., et al. 2010. CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. Journal of Biological Chemistry, 285: 17930 – 1793. |
| 18819923 | 1:1000 | Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. Journal of Biological Chemistry, 283: 33138 - 33146. |
| 18519737 | not listed | Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831 - 846. |
Product Specific Protocols
- Western Blot Protocol: Download