Anti-GluR1-Subunit (Ser831) Antibody
Our Anti-GluR1-Subunit (Ser831) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat GluR1-Subunit (Ser831) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
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Ships: 1-2 business days
Product Specific References for Applications and Species
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 22427672 | not listed | Jakan-Sakrikar, N., et al. 2012. Substrate-selective and calcium-independent activation of CaMKII by α-actinin. Journal of Biological Chemistry, 287(19), pp.15275-15283. |
| 21610080 | not listed | Jiao, Y., et al. 2011. Characterization of a Central Ca2+/Calmodulin-dependent Protein Kinase IIα/β Binding Domain in Densin That Selectively Modulates Glutamate Receptor Subunit Phosphorylation. Journal of Biological Chemistry 286: 24806 - 24818. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 39341363 | 1:1000 | Gao, A.Y.L., et al. 2024. Impaired hippocampal plasticity associated with loss of recycling endosomal SLC9A6/NHE6 is ameliorated by the TrkB agonist 7,8-dihydroxyflavone. Biochimica et Biophysica Acta, 167529. |
| 37648853 | 1:2000 | Tullis, JE, et al. 2023. LTP induction by structural rather than enzymatic functions of CaMKII. Nature, 146-153. |
| 33853773 | 1:1000 | Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300. |
| 26255006 | 1:500 | Pasek, J.G., et al. 2015. Differential CaMKII regulation by voltage-gated calcium channels in the striatum. Molecular and Cellular Neuroscience, 68, 234-243. |
| 24907515 | not listed | Mao, L.M., et al. 2014. Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European Journal of Pharmacology, 738, 237-244. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. Journal Neuroscience, 30: 8263-8273. |
| 17923095 | not listed | Hu,H., et al. 2007. Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31994358 | 1:500 | Mao, L.M., et al. 2018. Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain. Brain and Behavior, p.e01543. |
| 29432883 | not listed | Mao, L.M., et al. 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93. |
| 29242119 | not listed | Xue, B., et al. 2017. Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European Journal of Pharmacology. 2017 Dec 11;820:45-52. |
| 28578480 | not listed | Lameth, J., et al. 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16. |
| 27060412 | not listed | Xue, B., et al. 2017. Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65. |
| 26706598 | not listed | O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of Disease, 87, 134-144. |
| 26658861 | 1:1000 | Bean, L.A., et al. 2015. Re-opening the critical window for estrogen therapy. Journal of Neuroscience, 35(49), pp.16077-16093. |
| 25689263 | 1:1000 | Mao, L.M., et al. 2015. Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of Neurochemistry. Jun;133(6):795-805. |
| 24780380 | 1:500 | Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12. |
| 24231469 | 1:1000 | Xue, B., et al. 2014. Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry International, 64, 48-54. |
| 24056996 | 1:1000 | Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Molecular Pharmacology, 84(6), 834-843. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. J. Neurosci., 30: 8263 - 8273. |
| 20353941 | not listed | Coultrap, S.J., et al. 2010. CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. Journal of Biological Chemistry, 285: 17930 – 1793. |
| 18819923 | 1:1000 | Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. Journal of Biological Chemistry, 283: 33138 - 33146. |
| 18519737 | not listed | Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831 - 846. |
Product Specific Protocols
- Western Blot Protocol: Download