Electron micrograph of L21/32 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) were observed in the postsynaptic densities of dendritic spines and dendritic shafts (Den) establishing asymmetrical synapses with axon terminals (b). Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
Anti-GluA2/GluR2 Glutamate Receptor Antibody
GluA2/GluR2 glutamate receptor
Glutelin type-A 2 (GluA2) , also called GRIA2, GLUR2, GLURB, GluA2, GluR-K2, HBGR2 or glutamate ionotropic receptor AMPA type subunit 2, is a member of the Glutamate receptor family of the mammalian brain. This neurotransmiter receptor subunit, which is activated during normal central nervous system fuction, is encoded by the GRIA2 (or GLUR2) gene. GluA2 constitutes a key subunit that regulates AMPA receptors. AMPA receptors lacking of Glua2 have been shown to be present in diseased brains, whose basic fuctions have been altered.
EM, IHC, WB
Fusion protein amino acids 834-883 (EFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI, cytoplasmic C-terminus) of rat GluA2/GluR2 (also known as Glutamate receptor 2, AMPA-selective glutamate receptor 2, Glutamate receptor ionotropic AMPA 2, GluR-B, GluR-K2 and Gria2, accession number P19491); Mouse: 98% identity (49/50 amino acids identical); Human: 98% identity (49/50 amino acids identical); 100% identity between Flip and Flop isoforms; 70% identity with GluA3/GluR3 and less identity with GluA1/GluR1 and GluA4/GluR4
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results)
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.