Anti-GluA1/GluR1 Glutamate Receptor Antibody (N355/1)

Name: Anti-GluA1/GluR1 Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-327
Price: 394.0 USD
Availability: InStock

Our mouse GluA1/GluR1 glutamate receptor monoclonal antibody from NeuroMab is produced in-house from clone N355/1. It detects human, mouse, and rat GluA1/GluR1 glutamate receptor and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunofluorescence staining of 14 DIV cultured rat cortical neuron with N355/1. Image courtesy of Brad Elmer and Kim McAllister (UCD).

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged GluR1, GluR2 or Kv2.1 plasmid and probed with N355/1 (left), L21/32 (middle) or N52A/42 (right) TC supe.

Western blot analysis of hippocampal synaptic receptor expression in 6 month old PS19 females and males mice showing a decrease in GluA1 (Cat. 75-327, 1:1000000) in females due to chronic sleep disruption (CSD). Image from publication, CC-BY-4.0. PMID: 38858068.

Immunofluorescence staining of 14 DIV cultured rat cortical neuron with N355/1. Image courtesy of Brad Elmer and Kim McAllister (UCD).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-327

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluA1/GluR1 glutamate receptor

Scientific Background

Glutamate receptor 1 or GluA1/GluR1 glutamate receptor is a member of the glutamate-gated ion channel family. GluR1 funcitons as an excitatory neurotransmitter at many synapses in the central nervous system and is widely expressed in brain. Mutations in the GluR1 gene are associated with cerebral cortical dysplasia, Status Epilepticus, and depression.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N355/1

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Gria1 Glur1

Molecular Weight

100 kDa

Immunogen

Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 (accession number P19490) produced recombinantly in E. Coli.

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315840

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

Does not cross-react with GluR2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

GLUR 1; Glutamate receptor 1; GLUR A; GluR-1; AMPA 1; AMPA-selective glutamate receptor 1; AMPA selective glutamate receptor 1; GluR-A; AMPA-selective glutamate receptor 1; GluR-K1; GluA1; Glutamate receptor ionotropic; GLUH1; GluA1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Glutamate receptor ionotropic; Glutamate receptor; ionotropic; Gria1; GRIA1_HUMAN; HBGR1; MGC133252; OTTHUMP00000160643; OTTHUMP00000165781; THUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; AMPA 1

UniProt ID

P19490
UniProt Summary: Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG2 or CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Resensitization is blocked by CNIH2 through interaction with CACNG8 in the CACNG8-containing AMPA receptors complex. Calcium (Ca(2+)) permeability depends on subunits composition and, heteromeric channels containing edited GRIA2 subunit are calcium-impermeable. Also permeable to other divalents cations such as strontium(2+) and magnesium(2+) and monovalent cations such as potassium(1+) and lithium(1+).

NCBI Gene ID

50592

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Anti-GluA1/GluR1 Glutamate Receptor Antibody (N355/1)

SKU: 75-327

Product Details

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