Anti-Gephyrin Antibody (L106/83)

Name: Anti-Gephyrin Antibody
Brand: NeuroMab
SKU: 75-443
Price: 329.0 USD
Availability: InStock

Our Anti-Gephyrin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L106/83. It detects human, mouse, and rat Gephyrin, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling

Adult rat cerebellum immunohistochemistry.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-443

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Gephyrin

Scientific Background

In neuronal tissue, gephyrin is a scaffolding protein that self assembles in a complex, flat submembraneous lattice that inhibits mobility of the glycine receptors (GlyR) and GABAA receptors (GABAAR) causing clustering at post synaptic sites (Groeneweg et al, 2018). In non-neuronal tissue gephyrin plays a critical role in the molybdendum cofactor (MoCo) biosynthesis of essential life molybdoenzymes, like sulphite oxidase (Groeneweg et al, 2018). Three functional domains have been identified in gephyrin: the stable, structural G and E domains, and the C domain which is intrinsically unstructured leading to multiple isoforms (108, 105, 102, 98, 90 kDa) (Kawasaki, et al 1997). The 93 kDa protein predominantly expressed in the brain and located in the plasma membrane, has a 10X stronger affinity for the GlyR than the GABAAR. Gephyrin’s flexibility to change its size and molecular density is directly correlated to its high affinity to the GlyR-β subunit, and is required for anchoring and accurate clustering of GlyRs at post synaptic sites and microtubule transport chains (Greoneweg et al, 2018). A consistent parameter in the pathogenesis of Alzheimers Disease shows a decrease of inhibitory GABAergic synapses and gephyrin, and increased levels of an insoluble 37 kDa gephyrin fragment not detected in healthy, non-AD models (Kiss et al, 2016).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L106/83

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GPHN GPH KIAA1385

Molecular Weight

80 kDa

Antigen

Fusion protein amino acids 1-181 (N-terminus) of human Gephyrin (accession number Q9NQX3) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2636851

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)]

UniProt ID

Q9NQX3

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
34576197	1:100	Kuljis, D.A., et al. 2021. Gephyrin-Lacking PV synapses on neocortical pyramidal neurons. International Journal of Molecular Science, 10032.
33704414	1:100	Micheva, K.D., et al. 2021. Conduction velocity along the local axons of parvalbumin interneurons correlates with the degree of axonal myelination. Cerebral Cortex, 3374-3392.
31554841	1:100	Simhal, A.K., et al. 2019. Multifaceted changes in synaptic composition and astrocytic involvement in a mouse model of fragile X syndrome. Scientific Reports, 13855.

Western Blot: Mouse
PMID	Dilution	Publication
37759527	not listed	Gruol, DL, et al. 2023. Impact of Elevated Brain IL-6 in Transgenic Mice on the Behavioral and Neurochemical Consequences of Chronic Alcohol Exposure. Cells, 0.
36775097	not listed	Gruol, D.L., et al. 2023. Neuroimmune Interactions with Binge Alcohol Drinking in the Cerebellum of IL-6 Transgenic Mice. Neuropharmacology, 109455.
35789851	1:1000	Huo, Y., et al. 2022. Prkn Knockout Mice Show Autistic-like Behaviors and Aberrant Synapse Formation. iScience, 104573.
32634532	not listed	Gruol, D.L., et al. 2020. Alcohol and IL-6 Alter Expression of Synaptic Proteins in Cerebellum of Transgenic Mice With Increased Astrocyte Expression of IL-6. Neuroscience, 124-137.
32202499	1:5000	Zhang, R.S., et al. 2020. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. Elife, e54443.
29787738	not listed	Gruol, D.L., et al. 2018. Altered Brain Activity During Withdrawal from Chronic Alcohol is Associated with Changes in IL-6 Signal Transduction and GABAergic Mechanisms in Transgenic Mice with Increased Astrocyte Expression of IL-6. Neuropharmacology., 32-46.
29787738	not listed	Gruol, D.L., et al. 2018. Altered brain activity during withdrawal from chronic alcohol is associated with changes in IL-6 signal transduction and GABAergic mechanisms in transgenic mice with increased astrocyte expression of IL-6. Neuropharmacology, 32-46.

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Anti-Gephyrin Antibody (L106/83)

SKU: 75-443

Product Details

Product Specific References for Applications and Species

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