Anti-Gephyrin Antibody (L106/83)

Name: Anti-Gephyrin Antibody
Brand: NeuroMab
SKU: 75-443
Price: 285.0 USD
Availability: InStock

from NeuroMab Datasheet

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Clonality

Monoclonal

Applications

AT, ICC, IHC, WB

Host Species

Mouse

Gene Name

GPHN GPH KIAA1385

Molecular Weight

80 kDa

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Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling

Adult rat cerebellum immunohistochemistry.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

Product Details

Target

Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)]

Target Description

In neuronal tissue, gephyrin is a scaffolding protein that self assembles in a complex, flat submembraneous lattice that inhibits mobility of the glycine receptors (GlyR) and GABAA receptors (GABAAR) causing clustering at post synaptic sites (Groeneweg et al, 2018). In non-neuronal tissue gephyrin plays a critical role in the molybdendum cofactor (MoCo) biosynthesis of essential life molybdoenzymes, like sulphite oxidase (Groeneweg et al, 2018). Three functional domains have been identified in gephyrin: the stable, structural G and E domains, and the C domain which is intrinsically unstructured leading to multiple isoforms (108, 105, 102, 98, 90 kDa) (Kawasaki, et al 1997). The 93 kDa protein predominantly expressed in the brain and located in the plasma membrane, has a 10X stronger affinity for the GlyR than the GABAAR. Gephyrin’s flexibility to change its size and molecular density is directly correlated to its high affinity to the GlyR-ß subunit, and is required for anchoring and accurate clustering of GlyRs at post synaptic sites and microtubule transport chains (Greoneweg et al, 2018). A consistent parameter in the pathogenesis of Alzheimers Disease shows a decrease of inhibitory GABAergic synapses and gephyrin, and increased levels of an insoluble 37 kDa gephyrin fragment not detected in healthy, non-AD models (Kiss et al, 2016).

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L106/83

Antibody Isotype

IgG2b

Applications

AT, ICC, IHC, WB

Host Species

Mouse

Gene Name

GPHN GPH KIAA1385

Molecular Weight

80 kDa

Antigen

Fusion protein amino acids 1-181 (N-terminus) of human Gephyrin (also known as Molybdopterin molybdenumtransferase, MPT Mo-transferase, Domain G, Domain E, Putative glycine receptor-tubulin linker protein, GPHN, GPH and KIAA1385, accession number Q9NQX3); Mouse: 100% identity (181/181 amino acids identical); Rat: 100% identity (181/181 amino acids identical) with isoform 5, slightly lower with other isoforms

Antibody Registry ID

AB_2636851

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Conjugate

Unconjugated

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Gephyrin [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)]

UniProt Details

UniProt (Human): Q9NQX3
UniProt (Immunogen Species): Q9NQX3

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Anti-Gephyrin Antibody (L106/83)

Anti-Gephyrin Antibody
SKU: 75-443

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Anti-Gephyrin Antibody (L106/83)

Anti-Gephyrin Antibody SKU: 75-443

Product Details

Anti-Gephyrin Antibody
SKU: 75-443