Anti-Gephyrin Antibody (L106/83)

Name: Anti-Gephyrin Antibody
Brand: NeuroMab
SKU: 75-443
Price: 337.0 USD
Availability: InStock

Our mouse Gephyrin monoclonal antibody from NeuroMab is produced in-house from clone L106/83. It detects human, mouse, and rat Gephyrin and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA, AT.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

Adult rat cerebellum immunohistochemistry.

Array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/83 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-443

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Gephyrin

Scientific Background

In neuronal tissue, gephyrin is a scaffolding protein that self assembles in a complex, flat submembraneous lattice that inhibits mobility of the glycine receptors (GlyR) and GABAA receptors (GABAAR) causing clustering at post synaptic sites (Groeneweg et al, 2018). In non-neuronal tissue gephyrin plays a critical role in the molybdendum cofactor (MoCo) biosynthesis of essential life molybdoenzymes, like sulphite oxidase (Groeneweg et al, 2018). Three functional domains have been identified in gephyrin: the stable, structural G and E domains, and the C domain which is intrinsically unstructured leading to multiple isoforms (108, 105, 102, 98, 90 kDa) (Kawasaki, et al 1997). The 93 kDa protein predominantly expressed in the brain and located in the plasma membrane, has a 10X stronger affinity for the GlyR than the GABAAR. Gephyrin’s flexibility to change its size and molecular density is directly correlated to its high affinity to the GlyR-β subunit, and is required for anchoring and accurate clustering of GlyRs at post synaptic sites and microtubule transport chains (Greoneweg et al, 2018). A consistent parameter in the pathogenesis of Alzheimers Disease shows a decrease of inhibitory GABAergic synapses and gephyrin, and increased levels of an insoluble 37 kDa gephyrin fragment not detected in healthy, non-AD models (Kiss et al, 2016).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L106/83

Isotype

IgG2b

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

GPHN GPH KIAA1385

Molecular Weight

80 kDa

Immunogen

Fusion protein amino acids 1-181 (N-terminus) of human Gephyrin (accession number Q9NQX3) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2636851

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Domain E; Gephyrin [Includes: Molybdopterin adenylyltransferase; Domain G; MPT adenylyltransferase; GEPH; EC 2.7.7.75; GEPH_HUMAN; Domain G; GPH; Molybdopterin molybdenumtransferase; GPHN; MPT Mo-transferase; GPHRYN; EC 2.10.1.1; KIAA1385; Domain E; Molybdopterin molybdenumtransferase; MPT adenylyltransferase; MPT Mo-transferase

UniProt ID

Q9NQX3
UniProt Summary: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.

NCBI Gene ID

10243

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Anti-Gephyrin Antibody (L106/83)

SKU: 75-443

Product Details

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