Anti-E-Cadherin (Cytoplasmic) Antibody (M168)

Name: Anti-E-Cadherin (Cytoplasmic) Antibody
Brand: PhosphoSolutions
SKU: CM1681
Price: 360.0 USD
Availability: InStock

10 Citations

Our E-Cadherin (Cytoplasmic) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat E-Cadherin (Cytoplasmic) and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Western blot image of human A431 cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). Blots were probed with anti-E-Cadherin (Cytoplasmic) and anti-N-Cadherin (Tyr-860)/E-Cadherin (Tyr-835) conserved site.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

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SKU: CM1681

Volume: 100 µL

Variant

Price:

$360.00

Quantity:

Product Details

Target

E-Cadherin (Cytoplasmic)

Target Description

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M168

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CDH1

Molecular Weight

120

Antigen

Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:50
ICC: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Uvomorulin, Cadherin-1, CTF1, CTF2, CTF3, CD324, Epithelial Cadherin

UniProt Details

UniProt (Human): P12830
UniProt (Immunogen Species): P09803

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse | Rat
Immunohistochemistry: Human | Mouse | Zebrafish
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
37556558	not listed	Peters, DE, et al. 2023. A gut-restricted glutamate carboxypeptidase II inhibitor reduces monocytic inflammation and improves preclinical colitis. Science Translational Medicine, eabn7491.
35549026	not listed	Eftekharjoo, M, et al. 2022. Epithelial Cell-Like Elasticity Modulates Actin-Dependent E-Cadherin Adhesion Organization. ACS biomaterials science &engineering, 2455-2462.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
33820817	not listed	Hennessey, C, et al. 2021. Neonatal Enteropathogenic Escherichia coli Infection Disrupts Microbiota-Gut-Brain Axis Signaling. Infection and immunity, e0005921.
32640237	1:2500	Jackson, ND, et al. 2020. Single-Cell and Population Transcriptomics Reveal Pan-epithelial Remodeling in Type 2-High Asthma. Cell reports, 107872.

Immunocytochemistry: Rat
PMID	Dilution	Publication
35447047	1:50	Liu, D, et al. 2015. Identification and characterization of mesenchymal-epithelial progenitor-like cells in normal and injured rat liver. The Americal journal of pathology, 110-128.

Immunohistochemistry: Human
PMID	Dilution	Publication
35608904	1:2000	Kotas, ME, et al. 2022. IL-13–programmed airway tuft cells produce PGE2, which promotes CFTR-dependent mucociliary function. JCI insight, e159832.
34855273	1:200	Zenner, ML, et al. 2022. Advanced glycation end‐products (AGEs) are lower in prostate tumor tissue and inversely related to proportion of West African ancestry. The Prostate, 306-313.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35359925	1:200	Zangara, MT, et al. 2022. Maltodextrin consumption impairs the intestinal mucus barrier and accelerates colitis through direct actions on the epithelium. Frontiers in immunology, 841188.

Immunohistochemistry: Zebrafish
PMID	Dilution	Publication
20405038	not listed	Tay, HG, et al. 2010. A vertebrate-specific Chp-PAK-PIX pathway maintains E-cadherin at adherens junctions during zebrafish epiboly. PLoS one, e10125.

Western Blot: Human
PMID	Dilution	Publication
25705818	1:1000	Signorelli, P, et al. 2015. Natural grape extracts regulate colon cancer cells malignancy. Nutrition and cancer, 494-503.

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Anti-E-Cadherin (Cytoplasmic) Antibody (M168)

SKU: CM1681

Product Details

Product Specific References for Applications and Species

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