Our Anti-Connexin 43 Antibody primary antibody from PhosphoSolutions is rabbit polyclonal. It detects rat Connexin 43 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43 kDa connexin43 protein.
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, IHC, WB
Rabbit
GJA1
43 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region or rat connexin43, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:200
Unconjugated
Specific for endogenous levels of the ~43 kDa connexin43 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43; Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3; Gap junction Protein Alpha 1 43kDa
P08050
UniProt Summary: Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells (By similarity). Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (Probable). Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in the conductive system of ventricular myocardium and heart morphogenesis (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (By similarity). Involved in intercellular innate immune signaling (PubMed:24077100, PubMed:31992625, PubMed:40010341). Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (PubMed:24077100, PubMed:31992625, PubMed:40010341). {ECO:0000250|UniProtKB:P08050, ECO:0000250|UniProtKB:P23242, ECO:0000269|PubMed:24077100, ECO:0000269|PubMed:31992625, ECO:0000269|PubMed:40010341, ECO:0000305|PubMed:11741837}.
