Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

Name: Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody
Brand: Aves Labs
SKU: CNP
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

2 Citations

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

CNP

Molecular Weight

47 kDa

Go to Full Product Details

CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.

A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.

Volume: 1000 µL

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Price:

$440

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Bulk Order Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

Product Details

Target

Murine CNPase (EC 3.1.4.37)

Target Description

Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

CNP

Molecular Weight

47 kDa

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313538

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 3.1.4.37

UniProt Details

UniProt (Immunogen Species): P09543

Beirowski B, Wong KM, Babetto E, Milbrandt J. (2017), 'mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells .' PNAS. 10.1073/pnas.1620761114.
Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.

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Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.