Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.
Primary Antibody
Human, Mouse, Rat
ICC, IHC, WB
Chicken
TUBB3 TUBB4
50 kDa






Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).
- Göttle P, Sabo JK, Heinen A, Venables G, Torres K, Tzekova N, Parras CM, Kremer D, Hartung HP, Cate HS, Küry P (2015), 'Oligodendroglial Maturation Is Dependent on Intracellular Protein Shuttling.' The Journal of Neuroscince. 10.1523/JNEUROSCI.1423-14.2015.
- Daadi MM, Klausner JQ, Bajar B, Goshen I, Lee-Messer C, Lee SY, Winge MC, Ramakrishnan C, Lo M, Sun G, Deisseroth K, Steinberg GK (2016), 'Optogenetic Stimulation of Neural Grafts Enhances Neurotransmission and Downregulates the Inflammatory Response in Experimental Stroke Model.' Cell Transplantation. 10.3727/096368915X688533.
- Vuong TA, Leem YE, Kim BG, Cho H, Lee SJ, Bae GU, Kang JS (2016), 'A Sonic hedgehog coreceptor, BOC regulates neuronal differentiation and neurite outgrowth via interaction with ABL and JNK activation.' Cellular Signalling. 10.1016/j.cellsig.2016.11.013.
- Nagai T, Ikegami Y, Mizumachi H, Shirakigawa N, Ijima H (2017), 'Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture.' Journal of Bioscience and Bioengineering. 10.1016/j.jbiosc.2017.04.018.
- Wang C, Ward ME, Chen R, Liu K, Tracy TE, Chen X, Xie M, Sohn PD, Ludwig C, Meyer-Franke A, Karch CM, Ding S, Gan L (2017), 'Scalable Production of iPSC-Derived Human Neurons to Identify Tau-Lowering Compounds by High-Content Screening.' Stem Cell Reports. 10.1016/j.stemcr.2017.08.019.
- Yap CC, Digilio L, McMahon L, Winckler B (2017), 'The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.' Scientific Reports. 10.1038/s41598-017-07667-x.
- Bigler RL, Kamande JW, Dumitru R, Niedringhaus M, Taylor AM (2017), 'Messenger RNAs localized to distal projections of human stem cell derived neurons.' Nature Scientific Reports. 10.1038/s41598-017-00676-w.
- Al-Gharaibeh A, Culver R, Stewart AN, Srinageshwar B, Spelde K, Frollo L, Kolli N, Story D, Paladugu L, Anwar S, Crane A, Wyse R, Maiti P, Dunbar GL, Rossignol J (2017), 'Induced Pluripotent Stem Cell-Derived Neural Stem Cell Transplantations Reduced Behavioral Deficits and Ameliorated Neuropathological Changes in YAC128 Mouse Model of Huntington's Disease.' Frontiers in Neuroscience. 10.3389/fnins.2017.00628.
- Nagendran T, Larsen RS, Bigler RL, Frost SB, Philpot BD, Nudo RJ, Taylor AM (2017), 'Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling.' Natrue Communications. 10.1038/s41467-017-00652-y.
- Göttle P, Manousi A, Kremer D, Reiche L, Hartung HP, Küry P (2018), 'Teriflunomide promotes oligodendroglial differentiation and myelination.' Journal of Neuroinflammation. 10.1186/s12974-018-1110-z.
- Muñoz Y, Paula-Lima AC, Núñez MT (2018), 'Reactive oxygen species released from astrocytes treated with amyloid beta oligomers elicit neuronal calcium signals that decrease phospho-Ser727-.' Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2018.01.006.
- Morton MC, Neckles VN, Seluzicki CM, Holmberg JC, Feliciano DM (2018), 'Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen.' Cell Reports. 10.1016/j.celrep.2018.03.037.
- J. J. Brudvig, J. T. Cain, R. M. Sears, G. G. Schmidt-Grimminger, E. S. Wittchen, K. B. Adler, H. T. Ghashghaei, J. M. Weimer (2018), 'MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network.' Nature Scientific Reports. 10.1038/s41598-018-31578-0.
- DeVos SL, Corjuc BT, Oakley DH, Nobuhara CK, Bannon RN, Chase A, Commins C, Gonzalez JA, Dooley PM, Frosch MP, Hyman BT (2018), 'Synaptic Tau Seeding Precedes Tau Pathology in Human Alzheimer's Disease Brain.' Frontiers in Neuroscience. 10.3389/fnins.2018.00267.
- Yap CC, Digilio L, McMahon LP, Garcia ADR, Winckler B (2018), 'Degradation of dendritic cargos requires Rab7- dependent transport to somatic lysosomes.' Journal of Cell Biology. 10.1083/jcb.201711039.
- Anderson EM, Larson EB, Guzman D, Wissman AM, Neve RL, Nestler EJ, Self DW (2018), 'Overexpression of the Histone Dimethyltransferase G9a in Nucleus Accumbens Shell Increases Cocaine Self-Administration, Stress-Induced Reinstatement, and Anxiety.' The Journal of Neuroscince. 10.1523/JNEUROSCI.1657-17.2017.
- Yamada S, Sakakibara SI (2018), 'Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system.' Journal of Comparative Neurology. 10.1002/cne.24495.
- Göttle P, Förster M, Gruchot J, Kremer D, Hartung HP, Perron H, Küry P (2018), 'Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination.' Glia. 10.1002/glia.23535.
- Jian Dong, Yuan-Bo Pan, Xin-Rong Wu, Li-Na He, Xian-Dong Liu, Dong-Fu Feng, Tian-Le Xu, Suya Sun, Nan-Jie Xu (2019), 'A neuronal molecular switch through cell-cell contact that regulates quiescent neural stem cells.' Science Advances. 10.1126/sciadv.aav4416.
- Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, A. Denise R. Garcia, and Bettina Winckler (2018), 'Degradation of dendritic cargos requires Rab7- dependent transport to somatic lysosomes.' The Journal of Cell Biology. 10.1083/jcb.201711039.
- Diego Pulzatto Cury PhD, Barbara Tavares Schafer PhD, Sonia Regina Yokomizo de Almeida BSc, Marta Maria da Silva Righetti BSc, Ii-sei Watanabe PhD (2019), 'Application of a Purified Protein From Natural Latex and the Influence of Suture Type on Achilles Tendon Repair in Rats.' The American Journal of Sports Medicine. 10.1177/0363546518822836.
- Xiao Xiao, Raj Putatunda, Yonggang Zhang, Priya V. Soni, Fang Li, Ting Zhang, Mingyang Xin, Jin Jun Luo, John R. Bethea, Yuan ChengEmail author and Wenhui Hu (2018), 'Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells.' Journal of Neuroinflammation. 10.1186/s12974-018-1074-z.