Name: Anti-Beta-Tubulin 3 Antibody
Brand: Aves Labs
SKU: TUJ
Price: 410.0 USD
Availability: InStock

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

TUBB3 TUBB4

Molecular Weight

50 kDa

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Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-actin (Rockland, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Aves Labs, F-1005, 1:1000 dilution); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.

β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000 dilution). Hoda Ilias, Aves Labs.

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

Volume: 1000 µL

Variant

Price:

$410.00

Quantity:

Product Details

Target

Beta-Tubulin 3

Target Description

Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

TUBB3 TUBB4

Molecular Weight

50 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313564

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III)

UniProt Details

UniProt (Immunogen Species): Q13509

Citations (20)

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Göttle P, Manousi A, Kremer D, Reiche L, Hartung HP, Küry P (2018), 'Teriflunomide promotes oligodendroglial differentiation and myelination.' Journal of Neuroinflammation. 10.1186/s12974-018-1110-z.
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Jian Dong, Yuan-Bo Pan, Xin-Rong Wu, Li-Na He, Xian-Dong Liu, Dong-Fu Feng, Tian-Le Xu, Suya Sun, Nan-Jie Xu (2019), 'A neuronal molecular switch through cell-cell contact that regulates quiescent neural stem cells.' Science Advances. 10.1126/sciadv.aav4416.
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Anti-Beta-Tubulin 3 Antibody

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.