Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody

Name: Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP2961
Price: 340.0 USD
Availability: InStock

2 Citations

Our β-Catenin (Tyr-489)[γ-Catenin (Tyr-480)] rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat β-Catenin (Tyr-489)[γ-Catenin (Tyr-480)] and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).

Immunocytochemical labeling of β-Catenin in pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-489) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: CP2961

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

β-Catenin (Tyr-489)[γ-Catenin (Tyr-480)]

Scientific Background

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

CTNNB1

Molecular Weight

84/88

Immunogen

Phospho-β-Catenin (Tyr-489) synthetic peptide corresponds to amino acid residues around tyrosine 489 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin, and has high homology to the conserved site in γ-Catenin (Tyr-480).

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-β-Catenin (Tyr-654) peptide before affinity purification using phospho-β-Catenin (Tyr-489) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of γ-Catenin and β-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-489

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Catenin beta1, CTNNB1, catenin

UniProt ID

P35222

Shipping

Blue Ice

Product Specific References for Applications and Species

ELISA: Mouse
Western Blot: Human

ELISA: Mouse
PMID	Dilution	Publication
21705496	1:500	Beard Jr, R.S., et al. 2011. Hyperhomocysteinemia increases permeability of the blood-brain barrier by NMDA receptor-dependent regulation of adherens and tight junctions. Blood, 2007-2014.

Western Blot: Human
PMID	Dilution	Publication
20671195	not listed	Funakoshi, S., et al. 2010. Intestine-specific transcription factor Cdx2 induces E-cadherin function by enhancing the trafficking of E-cadherin to the cell membrane. American Journal of Physiology. Gastrointestinal and Live Physiology, G1054-G1067.

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Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody

SKU: CP2961

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-β-Catenin (Tyr-489) [γ-Catenin (Tyr-480)], Phosphospecific Antibody

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