Anti-Asc (Tyr-144), Phosphospecific Antibody

Name: Anti-Asc (Tyr-144), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP5631
Price: 340.0 USD
Availability: InStock

12 Citations

Our Asc (Tyr-144) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Asc (Tyr-144) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.

Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University)

Immunoprecipitation analysis of ASC Y144 (Cat. AP5631) phosphorylation in mouse bone marrow-derived macrophages (BMDMs) infected with Mock, PRV-WT, PRV-UL4mut for 12 h. Image from publication CC-BY-4.0. Cat. AP5631, 1:1000). Image from publication, CC-BY-4.0. PMID: 38371945.

Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1 mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: AP5631

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Asc (Tyr-144)

Scientific Background

Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. Asc can be phosphorylated at Tyr-144 in a Syk and JNK-dependent manner. This phosphorylation is critical for Asc speck formation and Caspase-1 activation.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

Pycard

Molecular Weight

Immunogen

Asc (Tyr-144) phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-144 in mouse Asc. This peptide sequence is highly conserved in human and rat Asc.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated Asc (Tyr-144) peptide before affinity purification using phospho-Asc (Tyr-144) peptide. The antibody detects phosphorylated Asc (Tyr-144) in the inflammasome. In J774 macrophage cells primed with LPS and treated with nigericin, the antibody colocalized with an inflammasome marker, caspase-1 inhibitor (FAM-YVAD-FMK). In addition, the antibody detects a 20 kDa Asc protein in western blots of J774A.1 mouse macrophages treated with pervanadate.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-144

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Caspase, PYCARD, AIM, ASC, CARD5, TMS1

UniProt ID

Q9EPB4

Shipping

Blue Ice

Product Specific References for Applications and Species

ELISA: Mouse
Immunohistochemistry: Mouse
Western Blot: Human | Mouse

ELISA: Mouse
PMID	Dilution	Publication
32963032	not listed	Lin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32963032	not listed	Lin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.

Western Blot: Human
PMID	Dilution	Publication
33568399	not listed	Gavrilin, MA, et al. 2021. cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation. Journal of immunology (Baltimore, Md. : 1950), 1329-1336.
30157949	not listed	Furuya, MY, et al. 2018. Tofacitinib inhibits granulocyte-macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils. Arthritis research & therapy, 196.

Western Blot: Mouse
PMID	Dilution	Publication
39316625	not listed	Zhang, X., et al. 2024. Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation. PLoS Pathogens, e1012546.
38953285	1:1000	Kang, H, et al. 2024. ER-to-lysosome Ca2+ refilling followed by K+ efflux-coupled store-operated Ca2+ entry in inflammasome activation and metabolic inflammation. eLife, .
38371945	1:1000	Park, K, et al. 2023. A novel NLRP3 inhibitor as a therapeutic agent against monosodium urate-induced gout. Frontiers in Immunology, 1307739.
35474599	1:1000	Jung, ES, et al. 2022. Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway. Aging cell, e13623.
32963032	not listed	Lin, YH, et al. 2020. Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury. Molecular & cellular proteomics: MCP, 2030-2047.
31017981	1:1000	Dubois, H, et al. 2019. Nlrp3 inflammasome activation and Gasdermin D-driven pyroptosis are immunopathogenic upon gastrointestinal norovirus infection. PLoS pathogens, e1007709.
29686531	not listed	Kwak, SB, et al. 2018. Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation. Mediators of inflammation, 6054069.
24721477	not listed	Hoyt, LR, et al. 2016. Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation. Journal of immunology (Baltimore, Md. : 1950), 1322-1334.

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Anti-Asc (Tyr-144), Phosphospecific Antibody

SKU: AP5631

Product Details

Product Specific References for Applications and Species

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