Anti-Arpc1b/p41-Arc (C-terminal region) Antibody

Name: Anti-Arpc1b/p41-Arc (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AP4321
Price: 340.0 USD
Availability: InStock

3 Citations

Our Arpc1b/p41-Arc (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Arpc1b/p41-Arc (C-terminal region) and is antigen affinity purified. It is great for use in WB, IP, ICC.

Western blot of rat A7r5 (lane 1), human Jurkat (lane 2), rat PC12 (lane 3), human PC3 (lane 4), mouse C2C12 (lane 5), and human A431 cells (lane 6). The blot was probed with rabbit polyclonal anti-Arpc1b (C-terminal region).

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Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

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Data Sheet

SKU: AP4321

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Arpc1b/p41-Arc (C-terminal region)

Scientific Background

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

ARPC1B

Molecular Weight

Immunogen

Arpc1b/p41-Arc synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arpc1b. This peptide sequence is highly conserved in rat, mouse, and chicken Arpc1b, and has some homology to the conserved region in isoform Arpc1a.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and Jurkat cells, rat A7r5 and PC12 cells, and mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Arc41, p41, Arpc1b

UniProt ID

O15143

Shipping

Blue Ice

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
35222279	1:1000	Merz, KE, et al. 2022. Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis. Frontiers in endocrinology, 821849.

Western Blot: Mouse
PMID	Dilution	Publication
33388318	not listed	Zhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.

Western Blot: Rat
PMID	Dilution	Publication
28972183	not listed	Tunduguru, R, et al. 2017. The actin-related p41ARC subunit contributes to p21-activated kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells. The Journal of biological chemistry, 19034-19043.

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Anti-Arpc1b/p41-Arc (C-terminal region) Antibody

SKU: AP4321

Product Details

Product Specific References for Applications and Species

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