Anti-Argonaute 2 Antibody

Name: Anti-Argonaute 2 Antibody
Brand: PhosphoSolutions
SKU: AP5281
Price: 275.0 USD
Availability: InStock

6 Citations

Our Argonaute 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Argonaute 2 and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: AP5281

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

Argonaute 2

Target Description

Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

AGO2

Molecular Weight

Antigen

Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids from the central region of human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of Ago2 on SDS-PAGE immunoblots of human A431 cells and mouse recombinant Ago2 full length protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer

UniProt Details

UniProt (Human): Q9UKV8
UniProt (Immunogen Species): Q9UKV8

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
PLA: Human

Western Blot: Human | Mouse | Xenopus

Immunocytochemistry: Human
PMID	Dilution	Publication
37819702	not listed	Bridges, MC, et al. 2023. Actin-dependent recruitment of AGO2 to the zonula adherens. Molecular Biology of the Cell, ar129.
28877994	not listed	Kourtidis, A, et al. 2017. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. The Journal of cell biology, 3073-3085.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32272708	not listed	Nair-Menon, J, et al. 2020. Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer. International journal of molecular sciences, 2559.

PLA: Human
PMID	Dilution	Publication
36497003	1:100	Daulagala, AC, et al. 2022. ECM Substrates Impact RNAi Localization at Adherens Junctions of Colon Epithelial Cells. Cells, 3740.

Western Blot: Human
PMID	Dilution	Publication
28877994	not listed	Kourtidis, A, et al. 2017. Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. The Journal of cell biology, 3073-3085.

Western Blot: Mouse
PMID	Dilution	Publication
32272708	not listed	Nair-Menon, J, et al. 2020. Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer. International journal of molecular sciences, 2559.

Western Blot: Xenopus
PMID	Dilution	Publication
35748902	not listed	Ingelson-Filpula, WA, et al. 2022. MicroRNA biogenesis proteins follow tissue-dependent expression during freezing in Dryophytes versicolor. Journal of Comparative Physiology, 611-622.
29681008	1:1000	Hadj-Moussa, H, et al. 2018. Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains. Cellular and molecular life sciences : CMLS, 3635-3647.

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Anti-Argonaute 2 Antibody

SKU: AP5281

Product Details

Product Specific References for Applications and Species

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