Anti-Actin (Tyr-53), Phosphospecific Antibody

Name: Anti-Actin (Tyr-53), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP1671
Price: 340.0 USD
Availability: InStock

4 Citations

Our Actin (Tyr-53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Actin (Tyr-53) and is antigen affinity purified. It is great for use in WB, ICC.

Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).

Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).

Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

SKU: AP1671

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Actin (Tyr-53)

Scientific Background

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

ACTB

Molecular Weight

Immunogen

Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-53

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

UniProt ID

P60709

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Rat | Rat
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
21629247	not listed	Vonach, C, et al. 2011. NF-κB mediates the 12(S)-HETE-induced endothelial to mesenchymal transition of lymphendothelial cells during the intravasation of breast carcinoma cells. British journal of cancer, 263-271.

Immunocytochemistry: Rat
PMID	Dilution	Publication
27170127	not listed	Bertling, E, et al. 2016. Actin Tyrosine-53-Phosphorylation in Neuronal Maturation and Synaptic Plasticity. The journal of Neuroscience, 5299-5313.

Western Blot: Human
PMID	Dilution	Publication
21629247	not listed	Vonach, C, et al. 2011. NF-κB mediates the 12(S)-HETE-induced endothelial to mesenchymal transition of lymphendothelial cells during the intravasation of breast carcinoma cells. British journal of cancer, 263-271.

Western Blot: Mouse
PMID	Dilution	Publication
29691465	1:1000	Pryazhnikov, E, et al. 2018. Longitudinal two-photon imaging in somatosensory cortex of behaving mice reveals dendritic spine formation enhancement by subchronic administration of low-dose ketamine. Scientific reports, 6464.

Recover password

Create my account

Anti-Actin (Tyr-53), Phosphospecific Antibody

SKU: AP1671

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Actin (Tyr-53), Phosphospecific Antibody

Related Products

Recently Viewed