Our Anti-14-3-3 (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 14-3-3 (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58(cat. p1433-58, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
YWHAB
29 kDa
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:500
Unconjugated
Specific for endogenous levels of the ~29 kDa 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser58
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
14 3 3 antibody, 14 3 3 protein beta antibody, 14 3 3 protein beta/alpha antibody, 14 3 3 protein zeta antibody ,14 3 3 zeta antibody,14-3-3 protein beta/alpha antibody, 14-3-3 protein/cytosolic phospholipase A2 antibody, 1433B_HUMAN antibody, GW128 antibody, HS1 antibody, KCIP 1 antibody, KCIP-1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, N-terminally processed antibody, Protein 1054 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein delta polypeptide antibody, Tyrosine 3/tryptophan 5 -monooxygenase activation protein zeta polypeptide antibody, YWHAB antibody, YWHAD antibody, YWHAZ antibody
UniProt (Immunogen Species): P35213
56011
Product Specific References for Applications and Species
Yang, W., et al. 2008. Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 - 2494.
Yang, W., et al. 2008. Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 - 2494.
