Hybridoma mAb Scale-Up

We offer two scale-up methods.

Once a mouse monoclonal cell line has been sub-cloned to monoclonality, it can be used as an infinite source of monoclonal antibody (mAb) through either in vitro (bioreactor) or in vivo (ascites) production.

Method Overview

In Vitro Bioreactor Production

Cell-line Expansion (1-2 weeks)

  • Monoclonal cell line is thawed and expanded for seeding bioreactor.
  • Cell-line adaptation to serum-free medium available upon request (adaptation will take up to 3 additional weeks).

Bioreactor Production (2 weeks)

  • Cell line is seeded into bioreactor and antibody produced.

mAb Purification

Pricing: Starts at $1,600


In Vivo Ascites Production

Cell-line Expansion (Project-dependent timeline)

  • Monoclonal cell line is thawed and expanded for seeding mice.

Inoculation (3-4 weeks)

  • Mice are primed, cells seeded, then antibody harvested.

mAb Purification (3 weeks)

Pricing: Starts at $1,300


Ready to Get Started?

To receive a consultation and quote from one of our scientists, submit your project details below. We will respond within two business days. If you require immediate assistance, call us at (800) 824-8540.

Thanks for your inquiry. The information you submitted has been sent to our Antibody Services Group. Typically, the team reviews submitted requests for 1-2 business days before responding so that we may offer substantial guidance from our first contact. If you need an immediate response, feel free to contact us at 1 (800) 824-8540. We look forward to working with you!
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