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Western blot of lysed HFF cells that were serum starved for 24 hours and then refed for 30 minutes showing specific immunolabeling of the ~130-260 kDa CaSR protein.
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Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and  immunofluorescent images (right).
 Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ
Western Blot of CD4+ cells showing specific immunolabeling of CD28.
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M007). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (CM0371) at 1:1000.Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (CM0371). The antibody was detected using goat anti-mouse Ig:DyLight® 594.
Native western blot of human BEAS-2B lung cells (lane 1), NCI-H446 lung cancer cells (lane 2), lung mesothelioma cells: NCI-H28 (lane 3), and NCI-H2052 (lane 4). The blot was probed with mouse monoclonal anti-CD47 (CM0541) at 1:500.Immunocytochemical labeling of CD47 in aldehyde fixed human MCF7 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-CD47 (CM0541). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0021). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55
extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M011). The antibody was detected using goat anti-mouse Ig DyLight® 594.
Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M015). The antibody was detected using goat anti-mouse Ig DyLight® 594.
Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0341). The antibody was detected using goat anti-mouse Ig DyLight® 594.Representative Standard Curve using mouse monoclonal anti-CD63 (CM0341) for ELISA capture of human recombinant CD63 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight® 594.

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