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Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti- SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho- serine peptide (lane 6).
PhosphoSolutions Anti-SHP1 (C-terminal region) Antibody
$285
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).
PhosphoSolutions Anti-SHP1 (Ser-591), Phosphospecific Antibody
$340
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).
PhosphoSolutions Anti-SHP1 (Tyr-536), Phosphospecific Antibody
$340
Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).
PhosphoSolutions Anti-SHP2 (N-terminal region) Antibody
$285
Western Blot of 50 ug of mouse brain lysate showing specific immunolabeling of SHPRH.
PhosphoSolutions Anti-SHPRH Antibody
$445
Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).Formalin fixed, citric acid treated parafin sections of mouse cerebral cortex. Sections were probed with anti-Slingshot-1L (SP1711) then anti-Rabbit:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Slingshot-1L (C-terminal region) Antibody
$340
Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1L (C-term.) and anti-SSH1L (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).
PhosphoSolutions Anti-Slingshot-1L (Ser-978), Phosphospecific Antibody
$340
Western blot analysis of SMAD7 in human HepG2 cells. The blot was probed with rabbit polyclonal SMAD7 antibody (SP4901) at 1:250.
PhosphoSolutions Anti-SMAD7 (N-terminal region) Antibody
$285
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25 kDa SNAP25 phosphorylated at Ser187 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 400 units/100ul lysate for 30 minutes, RT).
PhosphoSolutions Anti-SNAP25 (Ser187) Antibody
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Western blot of rat cortical lysate showing specific labeling of the ~25 kDa SNAP25 protein.
PhosphoSolutions Anti-SNAP25 Antibody
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Western blot image of human Sox2 recombinant protein untreated (lanes 1 & 3) or treated with Set7 methyltransferase to methylate Lys-119 (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific antibody (lanes 3 & 4).Immunocytochemical labeling of methylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Lys-119) methyl-specific (SP5531). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Sox2 (Lys-119), methyl-specific Antibody
$340
Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Sox2 (Thr-118), Phosphospecific Antibody
$340
Western blot of mouse F9 stem cells untreated (lanes 1 & 3) and treated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 antibody (lanes 1 & 2) and rabbit polyclonal Sox2 (Thr-128) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-128) phospho-specific (SP0381). The antibody was detected using goat anti-rabbit Ig:DyLight® 594.
PhosphoSolutions Anti-Sox2 (Thr-128), Phosphospecific Antibody
$340
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.Immunofluoresence of FLAG-tagged SOX2 transfected COS cells showing SOX2 staining in red (Cat. 1907-SOX2, 1:20) and FLAG staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
PhosphoSolutions Anti-SOX2 Antibody
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Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Sox2 (SM5511). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Sox2 Antibody
$285
Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human SK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-SK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).Immunocytochemical labeling of sphingosine kinase 1 (SPHK1) in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-SPHK1 (SM5401). The antibody was detected using goat anti-rat DyLight® 594.
PhosphoSolutions Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein. Blots were probed with rabbit polyclonals anti-SK1 (Central Region) (SP1621) at 1:1000 (lane 1) and 1:4000 (lane 2), and anti-SK1 (C-terminal region) (SP5421) at 1:1000 (lane 3) and 1:4000 (lane 4).The protein expression levels of DGKα, SphK1 (Cat. SP5421, 1:1000) and p-SphK1(Ser225) (Cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305
PhosphoSolutions Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).
PhosphoSolutions Anti-Sphingosine Kinase 1 (Central region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).
PhosphoSolutions Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4). The blots were probed with rabbit polyclonal anti-SK2 (N-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-SK2 (N-terminal region) at 1:250 (lane 3) or 1:1000 (lane 4).
PhosphoSolutions Anti-Sphingosine Kinase 2 (N-terminal region) Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Sphingosine Kinase 2 (N-terminal region) Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Sphingosine Kinase 2 (Thr-578), Phosphospecific Antibody
$340
Western blot of rat synaptic plasma membrane (SPM) lysate showing specific immunolabeling of the ~36 kDa stargazin protein.
PhosphoSolutions Anti-Stargazin Antibody
From $160
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Western blot analysis of A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
PhosphoSolutions Anti-Stat1 (Tyr-701), Phosphospecific Antibody
$340

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