PhosphoSolutions

Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Representative Standard Curve using mouse monoclonal antiL1CAM (LM0231) for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

PhosphoSolutions Anti-L1CAM (Extracellular) Antibody

$370

Immunostaining of HeLa cells showing strong nuclear lamina staining with anti-Lamin A/C (Cat. 1027-LAM, red, 1:1000) and anti-Vimentin (Cat. 2105-VIM, green, 1:500).

Western blot of HeLa cell lysate showing specific immunolabeling of the ~64 kDa and ~74 kDa lamin A/C proteins.

PhosphoSolutions Anti-Lamin A/C Antibody

From $160

Native western blot image of human laminin isoforms: laminin 521 (α5β2γ1), laminin 121 (α1β2γ1), laminin 221 (α2β2γ1), laminin 332 (α3β3γ2), laminin 511 (α5β1γ1), laminin 411 (α4β1γ1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin β2/γ1 subunit (LM0461) at 1:1000.

Immunocytochemical labeling of laminin β2/γ1 subunits in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Laminin β2/γ1 subunits (LM0461). The antibody was detected using goat anti-mouse DyLight® 594.

PhosphoSolutions Anti-Laminin β2/γ1 Subunits Antibody

$340

Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (Cat. 1030-LAMP1, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (Cat. 2105-VIM, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~100 kDa LAMP1 protein.

PhosphoSolutions Anti-LAMP1 Antibody

From $160

Western blot analysis of leupaxin in human PC-3, rat A7r5, and human A431 cells. The blot was probed with anti-Leupaxin (N-terminal region) at 1:1000 (Lanes 1-3). Anti-Actin molecular weight standard is shown in lane 4.

PhosphoSolutions Anti-Leupaxin (N-terminal region) Antibody

$285

Western blot image of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-term.) (lanes 1 & 2) and anti-LIMK1 (Thr-508) (lanes 3 & 4).

PhosphoSolutions Anti-LIMK1 (C-terminus) Antibody

$285

Western blot image of human A431 cells untreated (lanes 1 & 3) or treated (lanes 2 & 4)with calyculin A (100 nM for 30 min) . The blots were probed with anti-LIMK1 (C-terminus) (lanes 1 & 2) or anti-LIMK1 (Ser-323) (lanes 3 & 4).

PhosphoSolutions Anti-LIMK1 (Ser-323) [LIMK2 (Ser-314)], Phosphospecific Antibody

$340

PhosphoSolutions Anti-LIMK1 (Thr-508) [LIMK2 (Thr-505], Phosphospecific Antibody

$340

PhosphoSolutions Anti-LRRK2 Antibody

$445

PhosphoSolutions Anti-LRRK2 Antibody

$445

Western Blot showing specific immunolabeling of Ly-6K in KK47 human bladder carcinoma cells, LY-6K siRNA, but not scrambled siRNA knocked down Ly-6K expression. A GAPDH antibody was used as a protein loading control. Data courtesy of Masuda et al.

Western Blot of Ly6K protein with GST-tag, non-transfected control HEK293T cells, HEK293T cells transfected with Ly6K-GFP construct showing specific immunolabeling of Ly6K.

PhosphoSolutions Anti-Ly-6k Antibody

$445

Western Blot of 293T cell lysate showing specific immunolabeling of MAP1.

PhosphoSolutions Anti-MAP1 Antibody

$445

Western blot of rat brain lysate showing specific immunolabeling of the ~87 kDa MARCKS protein phosphorylated at Ser152,156, in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

PhosphoSolutions Anti-MARCKS (Ser152,156) Antibody

From $160

Immunolabeling of HeLa cells showing specific labeling of the MARCKs protein (anti-MARCKS antibody, cat. 1107-MARCKS, red, 1:5000) and β-tubulin protein (green). Anti-MARCKS binds MARCKs protein expressed in the plasma membrane and cytoplasm. The blue is Hoechst staining the nuclear DNA.

Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.

PhosphoSolutions Anti-MARCKS Antibody

From $160

Immunostaining of HEK293 cells transfected with mCherry(red) and stained with anti-mCherry antibody (Cat. 1203-mCherry, green, 1:500) subsequently appear yellow. The nuclei stained blue (DAPI) distinguishes untransfected cells.

Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).

PhosphoSolutions Anti-mCherry Antibody

From $160

Western Blot of transfected HEK 293T cell lysate showing specific immunolabeling of MCT1.

PhosphoSolutions Anti-MCT1 Antibody

$445

Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).

PhosphoSolutions Anti-mDia1 (N-terminal region) Antibody

$340

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

Immunofluorescent images showing Parvalbumin (hair cells, red) and Diaph3 (Cat. DP3491, 1:400, magenta) expression in cryo-sectioned cochlea (organ of Corti area). The knockdown of the endogenous Stub1 in mouse inner ear leads to severe hearing loss. AAV-ie vector containing GFP tag was used to package Stub1-shRNA. The left ear of P3 mice were injected with AAV-ie via round window membrane (RWM). Image from publication, CC-BY-4.0. PMID: 39322015.

PhosphoSolutions Anti-mDia2 (C-terminal region) Antibody

$340

Western blot analysis of COS-7 cells expressing flag-tagged mDia2 (lanes 1-3) or mDia2 mutant (T1061A) (lane 4). The cells were untreated (lane 1) or treated with calyculin A (lanes 2 & 3). The blot was treated with alkaline phosphatase (lane 2), then probed with anti-mDia2 (Thr-1061) (upper blot) or anti-flag antibody (lower blot). (Image provided by Dr. Christopher Mack in the Dept. of Pathology at UNC Chapel Hill).

PhosphoSolutions Anti-mDia2 (Thr-1061), Phosphospecific Antibody

$340

Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).

PhosphoSolutions Anti-mDia3 (C-terminus) Antibody

$340

PhosphoSolutions Anti-mDia3 (Ser-196)) [conserved site], Phosphospecific Antibody

$340

Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).

Immunocytochemical labeling of MeCP2 in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (C-terminus) rabbit polyclonal antibody (MP4591) in the absence (left) or presence (right) of blocking peptide (MX4595). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-MeCP2 (C-terminus) Antibody

$340

Western blot of human PC3 cells treated with calyculin A (25 mM) for 15 min. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (C-terminus) (lanes 1 & 2) or anti-MeCP2 (Ser-421) (lanes 3 & 4).

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-421) rabbit polyclonal antibody (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-MeCP2 (Ser-421), Phosphospecific Antibody

$340

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-80) rabbit polyclonal antibody (MP4601) in the absence (left) or presence (right) of blocking peptide (MX4605). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

PhosphoSolutions Anti-MeCP2 (Ser-80), Phosphospecific Antibody

$340

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