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Immunohistochemistry of mouse inner ear frozen sections showing specific staining of GLUT10 at 1:200 dilution. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.Western Blot showing specific immunolabeling of GLUT10 in Lane 1: 10ug of protein from liver Lane 2: 10ug of protein from cochlea (E18) Lane 3: 10ug of protein from cochlea (P7) Lane 4: 10ug of protein from cochlea (P10) Lane 5: 10ug of protein from cochlea (P14) Lane 6: 10ug of protein from cochlea (P17) Lane 7: 10ug of protein from cochlea (P60) Lane 8: 10ug of protein from cochlea (1 Year).
Western blot of rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.
Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.
Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.
Western blot analysis of a human recombinant GSK3β N-terminal fragment (lanes 1 & 2) and endogenous GSK3β expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3β at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).Immunocytochemical labeling of GSK3β in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK3β (GM1311) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western Blot of HeLa cell lysate showing specific immunolabeling of HDAC10.

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