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Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr317 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-EphrinB (Tyr317) Antibody
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Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr331 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1 mg protein for 30 min).
PhosphoSolutions Anti-EphrinB (Tyr331) Antibody
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 kDa ERK/MAPK protein.
PhosphoSolutions Anti-ERK/MAPK Antibody
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Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).Immunocytochemical labeling of ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal ERK1 (C-terminal region) and detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-ERK1 (C-terminal region) Antibody
$370
Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes 5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).
PhosphoSolutions Anti-ERK1(T207)/ERK2(T188) [conserved], Phosphospecific Antibody
$340
Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).Immunocytochemical labeling of phosphorylated ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rat A7r5 cells treated with calyculin A. The fixed cells were labeled with mouse monoclonal antibodies to anti-ERK1 (EM2331) and anti-ERK1/2 (Thr-202/Tyr-204) (EM2061). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488 (left) and DyLight® 594 (right).
PhosphoSolutions Anti-ERK1/2 (MAPK) (Thr-202/Tyr-204) [conserved], Phosphospecific Antibody
$370
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (Cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (Cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
PhosphoSolutions Anti-ERK1/2 (MAPK) (Thr202/Tyr204) Antibody
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Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes 5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).Immunocytochemical labeling of ERK2 in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-ERK2 (EP4071) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-ERK2 (central region) Antibody
$285
Western Blot of transfected full-length ESDN in 293T cells showing specific immunolabeling of ESDN/DCBLD2.
PhosphoSolutions Anti-ESDN Antibody
$445
Western Blot of T84 colon carcinoma cell lysate showing specific immunolabeling of ESE1. The antibody recognizes both isoforms of ESE1.
PhosphoSolutions Anti-ESE1 Antibody
$445
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).Immunocytochemical labeling of Estrogen Receptor α in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor α (EP5431). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Estrogen Receptor α (C-terminus) Antibody
$285
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody
$340
Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody
$340
Western Blot of mouse heart lysate showing specific immunolabeling of ETR3/CUGBP2.
PhosphoSolutions Anti-ETR3 (CUGBP2) Antibody
$445
Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.Immunofluorescence of HeLa cells showing clear localized labeling of the DNA in the nucleus with Anti-EWS (Cat. 540-EWS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:5000, red) labels the cytoplasmic intermediate filament, and Hoechst DNA stain (blue).
PhosphoSolutions Anti-EWS Antibody
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Western blot analysis of HUVECs untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). Blots were probed with mouse monoclonal anti-FAK (lanes 1 & 2) and anti-FAK (Tyr-397) (lanes 3 & 4).
PhosphoSolutions Anti-FAK (Tyr-397), Phosphospecific Antibody
$340
Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-FAM129B (Ser679, 683) Antibody
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser556 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
PhosphoSolutions Anti-FANCI (Ser556) Antibody
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Western blot of Hela cell lysate treated with UV (~254nm) for 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559.
PhosphoSolutions Anti-FANCI (Ser559) Antibody
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Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).Immunocytochemical labeling of Fascin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Fascin (FM2651). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Fascin (clone 55K2) Antibody
$285
Western blot analysis of mouse C2C12 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) mouse monoclonal (lanes 3 & 4).
PhosphoSolutions Anti-Fascin (Ser-39), Phosphospecific Antibody
$340
Western blot analysis of human HeLa cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
PhosphoSolutions Anti-Fascin (Ser-39), Phosphospecific Antibody
$340
PhosphoSolutions Anti-FBG2/FBXO6 Antibody
$445
Western Blot of 30 ug of recombinant human full length FBG3 protein showing specific immunolabeling of FBG3.
PhosphoSolutions Anti-FBG3 Antibody
$445

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