Anti-Centromere Protein Antibody
Our Anti-Centromere proteins human polyclonal primary antibody from Antibodies Incorporated is produced in-house. It detects Centromere proteins, and is purified by Protein A chromatography. It is great for use in ICC.
Immunolabeling of spreaded WT mouse pachytene spermatocytes positively labeling CREST/Centromere Protein (red, Cat 15-235, 1:20) and SYCP3 (green). Image kindly provided by Fernando Sánchez Sáez and Alberto M. Penás from Universidad de Salamanca, Spain.
Product Specific References for Applications and Species
- ELISA: Human
- Immunocytochemistry: Human | Mouse
- Immunohistochemistry: Fish | Gecko | Horse | Human | Mouse
- Western Blot: Human
- Additional Publications: Unspecified
| ELISA: Human | ||
| PMID | Dilution | Publication |
| 31682730 | 1:100 | van der Bijl, N., et al. 2019. Mutations in the stromal antigen 3 (STAG3) gene cause male infertility due to meiotic arrest. Human Reproduction, 2112-2119. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 37484914 | 1:200 | Didaskalou, S, et al. 2023. HURP Localization in Metaphase is the Result of a Multi-step Process Requiring its Phosphorylation at Ser627 Residue. Frontiers in cell and developmental biology, 981425. |
| 36414642 | 1:1000 | Zadra, I., et al. 2022. Chromosome Segregation Fidelity Requires Microtubule Polyglutamylation By The Cancer Downregulated Enzyme TTLL411. Nature Communications, 7147. |
| 36350697 | 1:500 | Janczyk, P.Ł, et al. 2022. Aurora A Phosphorylates Ndel1 To Reduce The Levels Of Mad1 And NuMA At Spindle Poles. Molecular Biology Of The Cell, . |
| 36322655 | not listed | Villarroya-Beltri, C., et al. 2022. Biallelic Germline Mutations In MAD1L1 Induce A Syndrome Of Aneuploidy With High Tumor Susceptibility. Science Advances, eabq5914. |
| 35858625 | not listed | Palmerola, K.L., et al. 2022. Replication Stress Impairs Chromosome Segregation and Preimplantation Development in Human Embryos. Cell, 2988-3007. |
| 35842428 | 1:2000 | De Marco Zompit, M., et al. 2022. The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis. Nature Communications, 4143. |
| 35776132 | 1:300 | Abad, M.A., et al. 2022. Mechanistic Basis for Sgo1-Mediated Centromere Localization and Function of the CPC. The Journal of Cell Biology, . |
| 35600766 | 1:40 | Hong, J., et al. 2022. Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression. Journal of Ginseng Research, 481-488. |
| 35483740 | 1:600 | Herman, J.A., et al. 2022. Functional dissection of human mitotic genes using CRISPR–Cas9 tiling screens. Genes and Development, 495-510. |
| 34797717 | 1:250 | Solon, A.L., et al. 2021. Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding. Science Advances, . |
| 34166371 | not listed | Hagihara, Y., et al. 2021. Tet1 regulates epigenetic remodeling of the pericentromeric heterochromatin and chromocenter organization in DNA hypomethylated cells. PLOS Genetics, e1009646. |
| 34048564 | not listed | Woodworth, M.A., et al. 2021. Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy. Nucleic Acids Research, e82. |
| 34048314 | not listed | Cheng, A., et al. 2021. Energy restriction causes metaphase delay and chromosome mis-segregation in cancer cells. Cell Cycle, 1195-1208. |
| 33981016 | 1:200 | Xu, X., et al. 2021. Targeting the actin/tropomyosin cytoskeleton in epithelial ovarian cancer reveals multiple mechanisms of synergy with anti-microtubule agents. British Journal of Cancer, 265-276. |
| 33619254 | 1:250 | Marquis, C., et al. 2021. Chromosomally unstable tumor cells specifically require KIF18A for proliferation. Nature Communications, 1213. |
| 33377866 | 1:600 | Herman, J.A., et al. 2020. chTOG is a conserved mitotic error correction factor. Elife, e61773. |
| 33180767 | 1:100 | Gil-Fernández, A., et al. 2020. Meiosis reveals the early steps in the evolution of a neo-XY sex chromosome pair in the African pygmy mouse Mus minutoides. PLOS Genetics, e1008959. |
| 32001712 | 1:40 | Kim, S., et al. 2020. PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation. Nature Communications, 612. |
| 31570499 | 1:300 | Abad, M.A., et al. 2019. Borealin–nucleosome interaction secures chromosome association of the chromosomal passenger complex. Journal of Cell Biology, 3912-3925. |
| 31420536 | 1:1000 | Han, T., et al. 2019. Interplay between c-Src and the APC/C co-activator Cdh1 regulates mammary tumorigenesis. Nature Communications, 3716. |
| 30709852 | 2.5ug/ml | Malaby, H.L., et al. 2019. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. Journal of Cell Biology, 1218-1234. |
| 30368549 | not listed | Yang, Y., et al. 2019. NudCL2 is an Hsp90 cochaperone to regulate sister chromatid cohesion by stabilizing cohesin subunits. Cellular and Molecular Life Sciences, 381-395. |
| 30143891 | not listed | Sun, M., et al. 2018. Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold. Chromosome Research, 277-295. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36429134 | 1:5 | Sánchez-Sáez, F., et al. 2022. The Oocyte-Specific Linker Histone H1FoOO Is Not Essential For Mouse Oogenesis And Fertility. Cells, . |
| 36354207 | 1:50 | Chen, L., et al. 2022. Septin 9 Controls Ccnb1 Stabilization Via APC/CCDC20 During Meiotic Metaphase I/Anaphase I Transition In Mouse Oocytes. Cell Proliferation, e13359. |
| 35805097 | 1:100 | Petrusová, J., et al. 2022. Focal adhesion protein Vinculin is required for proper meiotic progression during mouse spermatogenesis. Cells, 2013. |
| 35447083 | 1:500 | Mitrentsi, I., et al. 2022. Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations. Molecular cell, 2132-2147. |
| 35320753 | 1:500 | Chardon, F., et al. 2022. CENP-B-mediated DNA loops regulate activity and stability of human centromeres. Molecular cell, 1751-1767. |
| 34314679 | 1:500 | Chatzidaki, E.E., et al. 2021. Ovulation suppression protects against chromosomal abnormalities in mouse eggs at advanced maternal age. Current Biology, 4038-4051. |
| 32677148 | 1:200 | Hsieh, C., et al. 2020. MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA. EMBO Journal, e103614. |
| 32642764 | not listed | Mukaj, A., et al. 2020. Prdm9 Intersubspecific Interactions in Hybrid Male Sterility of House Mouse. Molecular Biology Evolution, 3423-3438. |
| 32634244 | 1:25 | Balboula, A.Z., et al. 2020. Vitrification-induced activation of lysosomal cathepsin B perturbs spindle assembly checkpoint function in mouse oocytes. Molecular Human Reproduction, 689-701. |
| 32574675 | 1:50 | Dong, F., et al. 2020. Chronic Cadmium Exposure Causes Oocyte Meiotic Arrest by Disrupting Spindle Assembly Checkpoint and Maturation Promoting Factor. Reproductive Toxicology, 141-149. |
| 32051254 | 1:5 | McNicoll, F., et al. 2020. Meiotic sex chromosome cohesion and autosomal synapsis are supported by Esco2. Life Science Alliance, e201900564. |
| 31895940 | not listed | Lovejoy, C.A., et al. 2020. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biology, e3000594. |
| 31750958 | 1:500 | Dumont, M., et al. 2020. Human chromosome‐specific aneuploidy is influenced by DNA‐dependent centromeric features. EMBO Journal, e102924. |
| 31495584 | not listed | Vargas-Hurtado, D., et al. 2019. Differences in mitotic spindle architecture in mammalian neural stem cells influence mitotic accuracy during brain development. Current Biology, 2993-3005. |
| 31492885 | 1:200 | Zhao, B., et al. 2019. ARID1A promotes genomic stability through protecting telomere cohesion. Nature Communications, 4067. |
| 31437213 | 1:5 | Gómez-H, L., et al. 2019. The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. PLOS Genetics, e1008316. |
| Immunohistochemistry: Fish (Lamprey) | ||
| PMID | Dilution | Publication |
| 36836858 | 1:100 | Matveevsky, S., et al. 2023. The First Analysis of Synaptonemal Complexes in Jawless Vertebrates: Chromosome Synapsis and Transcription Reactivation at Meiotic Prophase I in the Lamprey …. Life, 501. |
| Immunohistochemistry: Gecko | ||
| PMID | Dilution | Publication |
| 35388415 | 1:50 | Dedukh, D., et al. 2022. Premeiotic endoreplication is essential for obligate parthenogenesis in geckos. Development, . |
| Immunohistochemistry: Horse | ||
| PMID | Dilution | Publication |
| 33139583 | 1:250 | Rizzo, M., et al. 2020. The horse as a natural model to study reproductive aging-induced aneuploidy and weakened centromeric cohesion in oocytes. Aging, 22220-22232. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 35559990 | not listed | Kitasaka, H., et al. 2022. Oocyte cytoplasmic diameter of≥ 130 μm can be used to determine human giant oocytes. F&S Science, 10-17. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34573416 | 1:100 | Gil-Fernández, A., et al. 2021. Meiotic Behavior of Achiasmate Sex Chromosomes in the African Pygmy Mouse Mus mattheyi Offers New Insights into the Evolution of Sex Chromosome Pairing and …. Genes , 1434. |
| 33644894 | 1:50 | Mengoli, V., et al. 2021. Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension. EMBO Journal, e106812. |
| 33406415 | not listed | Cheng, E.C., et al. 2021. The essential function of SETDB1 in homologous chromosome pairing and synapsis during meiosis. Cell Reports, 108575. |
| 32555348 | 1:1000 | Bondarieva, A., et al. 2020. Proline-rich Protein PRR19 Functions With Cyclin-Like CNTD1 to Promote Meiotic Crossing Over in Mouse. Nature Communications, 3101. |
| 30674417 | 1:50 | Ehrmann, I., et al. 2019. An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. Elife, e39304. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 34731637 | 2ug/ml | Salinas-Luypaert, C., et al. 2021. Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-AK124ub. Cell Reports, 109924. |
| 31348889 | 1:500 | Logsdon, G.A., et al. 2019. Human artificial chromosomes that bypass centromeric DNA. Cell, 624-639. |
| 30635586 | 2ug/ml | Barra, V., et al. 2019. Phosphorylation of CENP-A on serine 7 does not control centromere function. Nature Communications, 175. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 35333353 | Zhong, Y., et al. 2022. XBP1 variant 1 promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6. Human Molecular Genetics, 2639-2654. | |
| 35194187 | Merchut-Maya, J.M., et al. 2022. Human cytomegalovirus hijacks host stress response fueling replication stress and genome instability. Cell Death and Differentiation, 1639-1653. | |
| 35146845 | Li, X., et al. 2022. Resveratrol protects renal damages induced by periodontitis via preventing mitochondrial dysfunction in rats. Oral Diseases, . | |
| 33825031 | Gil-Fernández, A., et al. 2021. Sex differences in the meiotic behavior of an XX sex chromosome pair in males and females of the mole vole Ellobius tancrei: turning an X into a Y chromosome?. Chromasoma, 113-131. | |
| 31940063 | Mihola, O.,, et al. 2020. Copy-number variation introduced by long transgenes compromises mouse male fertility independently of pachytene checkpoints. Chromasoma, 69-82. | |
| 30826871 | Ribagorda, M., et al. 2019. Meiotic behavior of a complex hexavalent in heterozygous mice for Robertsonian translocations: insights for synapsis dynamics. Chromasoma, 149-163. | |
| 30375749 | Hu, H., et al. 2019. Visualization of Genomic Loci in Living Cells with BiFC‐TALE. Current Protocols in Cellular Biology, e78. | |
| 30095409 | Frith, T.J., et al. 2018. Human axial progenitors generate trunk neural crest cells in vitro. Elife, e35786. | |
| 29805078 | Cánovas, B., et al. 2018. Targeting p38α increases DNA damage, chromosome instability, and the anti-tumoral response to taxanes in breast cancer cells. Cancer Cell, 1094-1110. | |
| 29718399 | Wu, X., et al. 2018. A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. Nucleic Acids Research, e80. | |
| 29670078 | Montero, J.J., et al. 2018. TERRA recruitment of polycomb to telomeres is essential for histone trymethylation marks at telomeric heterochromatin. Nature Communications, 1548. | |
| 29632243 | Berto, A., et al. 2018. Disentangling the molecular determinants for Cenp‐F localization to nuclear pores and kinetochores. EMBO Reports, e44742. | |
| 29618633 | Platani, M., et al. 2018. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. Journal of Cell Science, jcs213140. | |
| 29553540 | Hwang, G.H., et al. 2018. Chromatin spread preparations for the analysis of mouse oocyte progression from prophase to metaphase II. Journal of Visual Experiments, 56736. | |
| 29517484 | Lee, C.C., et al. 2018. Sumoylation promotes optimal APC/C activation and timely anaphase. Elife, e29539. | |
| 29513658 | Manterola, M., et al. 2018. BRDT is an essential epigenetic regulator for proper chromatin organization, silencing of sex chromosomes and crossover formation in male meiosis. PLOS Genetics, e1007209. | |
| 29443055 | Wellard, S.R., et al. 2018. A seminiferous tubule squash technique for the cytological analysis of spermatogenesis using the mouse model. Journal of Visual Experiments, 56453. | |
| 29423085 | Hanson, K., et al. 2018. The anti-rheumatic drug, leflunomide, synergizes with MEK inhibition to suppress melanoma growth. Oncotarget, 3815-3829. | |
| 29337080 | Biswas, U., et al. 2018. SMC1α substitutes for many meiotic functions of SMC1β but cannot protect telomeres from damage. Current Biology, 249-261. | |
| 28598437 | Guo, L.Y., et al. 2017. Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition. Nature Communications, 15775. | |
| 28539402 | Shirnekhi, H.K., et al. 2017. Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation. Molecular Biology Of The Cell, 2035-2041. | |
| 28469279 | Magescas, J., et al. 2017. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Scientific Reports, 1474. | |
| 28272472 | Sgourdou, P., et al. 2017. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Scientific Reports, 43708. | |
| 28263958 | Chia, G., et al. 2017. Genomic instability during reprogramming by nuclear transfer is DNA replication dependent. Nature Cell Biology, 282-291. | |
| 28252645 | Thole, T.M., et al. 2017. Neuroblastoma cells depend on HDAC11 for mitotic cell cycle progression and survival. Cell Death and Disease, e2635. | |
| 28186092 | Shumilov, A., et al. 2017. Epstein–Barr virus particles induce centrosome amplification and chromosomal instability. Nature Communications, 14257. | |
| 28074901 | Hu, H., et al. 2017. Live visualization of genomic loci with BiFC-TALE. Scientific Reports, 40192. | |
| 28050734 | Jordan, P.W., et al. 2017. Sororin is enriched at the central region of synapsed meiotic chromosomes. Chromosome Research, 115-128. | |
| 27918550 | Ly, P., et al. 2017. Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining. Nature Cell Biology, 68-75. | |
| 27614293 | Hamdane, N., et al. 2017. Disruption of the UBF gene induces aberrant somatic nucleolar bodies and disrupts embryo nucleolar precursor bodies. Gene, 5-11. | |