Anti-c-Abl (Tyr-412), Phosphospecific Antibody

Name: Anti-c-Abl (Tyr-412), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: AP1271
Price: 340.0 USD
Availability: InStock

2 Citations

Our c-Abl (Tyr-412) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat c-Abl (Tyr-412) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).

Representative ABL1p-Y412 (Cat. AP1271) immunohistochemistry of tumors or para-carcinom in human breat cancer tissue are shown. Image from publication CC-BY-4.0. PMID: 39060421

Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (AP1271; lanes 3 & 4), or anti-c-Abl (Tyr-245) (AP1251; lanes 5 & 6).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

SKU: AP1271

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

c-Abl (Tyr-412)

Scientific Background

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

ABL1

Molecular Weight

145

Immunogen

Phospho-c-Abl (Tyr-412) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-c-Abl (Tyr-245) peptide before affinity purification using phospho-c-Abl (Tyr-412) peptide. On SDS-PAGE immunoblots of K-562 treated with pervanadate the antibody detects a 145 kDa* protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-412

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

BCR-Abl, p150, Abelson murine leukemia viral oncogene homolog 1

UniProt ID

P00519

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunohistochemistry: Human
Western Blot: Human

Immunohistochemistry: Human
PMID	Dilution	Publication
39060421	not listed	Dong, Q., et al. 2024. ABL1-mediated phosphorylation promotes FOXM1-related tumorigenicity by Increasing FOXM1 stability. Cell Death and Differentiation, .
33107354	1:1000	Yang, YW, et al. 2021. Ponatinib is a potential therapeutic approach for malignant pleural mesothelioma. Experimental Lung Research, 45194.

Western Blot: Human
PMID	Dilution	Publication
39060421	not listed	Dong, Q, et al. 2024. ABL1-mediated phosphorylation promotes FOXM1-related tumorigenicity by Increasing FOXM1 stability. Cell death and differentiation, .
33107354	1:1000	Yang, YW, et al. 2021. Ponatinib is a potential therapeutic approach for malignant pleural mesothelioma. Experimental Lung Research, 45194.

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Anti-c-Abl (Tyr-412), Phosphospecific Antibody

SKU: AP1271

Product Details

Product Specific References for Applications and Species

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