Anti-Arl13b Recombinant Chicken Chimeric mAb (N295B/66)

Name: Anti-Arl13b Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-287
Price: 470.0 USD
Availability: InStock

Our chicken Anti-Arl13b recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N295B/66. It detects human, mouse, non-human primate, rat, and zebrafish Arl13b and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain lateral ventricle region showing cells stained by anti-Arl13b recombinant antibody (visualized in green) (Cat No. 78-287) at 1:100 dilution. The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat No. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-Arl13b specifically stains the ciliated cells surrounding the lateral ventricle. Magnification 400X.

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Species Reactivity

Human, Mouse, Non-Human Primate, Rat, Zebrafish

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: 78-287

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Arl13b

Scientific Background

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N295B/66

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Arl13b Arl2l1

Molecular Weight

50 kDa

Immunogen

Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Non-Human Primate, Rat, Zebrafish

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with Arl13a (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

UniProt ID

Q640N2

Shipping

Shipped on ice packs

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Anti-Arl13b Recombinant Chicken Chimeric mAb (N295B/66)

SKU: 78-287

Product Details

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