Myeloperoxidase Detection Kit

A sensitive kit to detect myeloperoxidase activity in neutrophils and macrophages as well as detecting PMN infiltration in tissue samples. Enables monitoring of multiple time points to follow kinetics and is adaptable to high throughput screening.

Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.
Figure 2. A MPO standard curve was prepared and run, as described in the protocol, in the absence (A) or presence (B) of 20 mM catalase inhibitor. MPO standard and reaction mix were added to a 96-well black plate and incubated at room temperature in the dark for 30 minutes. The wells were read using Ex: 530nm and Em: 590nm. There is an approximately 50% reduction in signal in the presence of 20mM catalase inhibitor (note the different scales on the x-axis).
Figure 3. Reaction mix was prepared without (A) and with (B) eosinophil peroxidase (EPO) inhibitor (1.0 mM final). Next MPO standard and reaction mix were added to individual wells of a 96-well black plate. The plate was incubated at room temperature in the dark for 30 minutes. Next the wells were read using Ex: 530nm and Em: 590nm. There is approximately 50% reduction in signal in the presence of the EPO inhibitor.
Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.
Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.
Click on image to zoom

Go to Full Product Details

Data Sheet
Protocol
Safety Data Sheet

SKU: FLMPO100-3

Size: 500 Tests
Price:
$522.00
Ships: 1-2 business days
  • Part# 4007: Detection Reagent, 1 Vial (-20C)
  • Part# 3002: 10X Assay Buffer, 60mL (2-8C)
  • Part# 3012: Hydrogen Peroxide, 1000µL of a Stabilized 3% Solution (2-8C)
  • Part# 6015: Myeloperoxidase, 1 Vial at 30Units/mL (2-8C)
  • Part# 4011: APF, 1 Vial (2-8C)

    • Bulk Order Myeloperoxidase Detection Kit

    Related Products

    Recently Viewed