Anti-Vimentin Antibody

Our Anti-Vimentin chicken polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Vimentin, and is IgY preparation. It is validated for use in ICC, IHC-Frozen, WB.

View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily Glial Fibrillary Acidic Protein (GFAP), or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
A view of neonatal rat brain cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (red) and with Rabbit polyclonal antibody to GFAP R-1374-50 (green). These two proteins are found only in non-neuronal cells so you can\'t see any neurons, except for their nuclei in blue. Maturish astrocytes have only GFAP so appear green or have a mix of both proteins, so appear yellow. Some cells only have the vimentin (immature astrocytes, microglia and fibroblasts) and so appear red.
Left: Analysis of vimentin expression in HeLa cells by Immunocytochemistry. Cells were stained with chicken antibody to vimentin (1:10,000, green), and co-stained with a mouse anti-actin antibody (red). Blue: DAPI nuclear stain. The vimentin antibody stains the intermediate filament network, while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites. Right: Western blot analysis of tissue and cell lysates using chicken antibody to Vimentin (1:5,000, red, lanes 2-6). [1] protein standard, [2] rat whole brain, [3] HeLa, [4] SH-SY5Y, [5] HEK293, [6] NIH-3T3. Vimentin protein appears as single band at around 50 kDa. The blot was simultaneously probed with a mouse antibody to MAP2C/D (green).
View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily Glial Fibrillary Acidic Protein (GFAP), or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
View of mixed neuron/glial cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily Glial Fibrillary Acidic Protein (GFAP), or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
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Human, Mouse, Rat
ICC, IHC, WB
Chicken

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Data Sheet

SKU: C-1409-50

Volume/Size: 50 µL
Price:
$327.00

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