Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) Antibody
Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) chicken polyclonal primary antibody detects bovine, human, mouse, other mammals (predicted), pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.
![Left: Analysis of UCHL1 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken antibody to UCHL1 (1:500, red). Cells were co-stained with a mouse antibody to vimentin (green). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells. Right: Western blot analysis of tissue and cell lysates using chicken antibody to UCHL1 (1:2,000, green), and a mouse anti-actin antibody (red, lanes 2-7). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties, but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.](http://www.antibodiesinc.com/cdn/shop/files/c-1406-50-ihc-wb_120x120.jpg?v=1759274035)
Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Ubiquitin C Terminal Hydrolase 1 C-1406-50 (green) and Rabbit polyclonal antibody to Glial Fibrillary Acidic Protein R-1374-50 (red). Blue is a DNA stain. Note that the Ubiquitin antibody stains neurons strongly and specifically and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
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