Anti-Ubiquitin Antibody
Our Anti-Ubiquitin rabbit polyclonal primary antibody detects human, mouse, and rat Ubiquitin, and is whole serum. It is validated for use in ICC, WB.
![Left: Immunofluorescent analysis of HeLa cells stained with rabbit antibody to ubiquitin (red, 1:1,000) by Immunocytochemistry. Cells were co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. [A] Control HeLa cells maintained in normal medium; [B] HeLa cells treated with 10 uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions [B]. Note the diffuse cytoplasmic ubiquitin staining in control cells [A] and well-defined ubiquitin positive inclusions in the Lc treated cells [B]. Right: Western blot analysis of HEK293 cell lysates using rabbit antibody to ubiquitin (green, 1:5,000). [1] protein standard, [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10 um for 16 hours. The blot was simultaneously probed with a mouse antibody to beta-tubulin as loading control (red, lanes 2 and 3). The smear detected above the 200 kDa standard represents accumulations of ubiquitinated proteins in the Lc-treated cells. The prominent band at ~8 kDa corresponds to monoubiquitin.](http://www.antibodiesinc.com/cdn/shop/files/r-1405-50-ihc-wb_120x120.jpg?v=1759274456)
![Left: Immunofluorescent analysis of HeLa cells stained with rabbit antibody to ubiquitin (red, 1:1,000) by Immunocytochemistry. Cells were co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. [A] Control HeLa cells maintained in normal medium; [B] HeLa cells treated with 10 uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions [B]. Note the diffuse cytoplasmic ubiquitin staining in control cells [A] and well-defined ubiquitin positive inclusions in the Lc treated cells [B]. Right: Western blot analysis of HEK293 cell lysates using rabbit antibody to ubiquitin (green, 1:5,000). [1] protein standard, [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10 um for 16 hours. The blot was simultaneously probed with a mouse antibody to beta-tubulin as loading control (red, lanes 2 and 3). The smear detected above the 200 kDa standard represents accumulations of ubiquitinated proteins in the Lc-treated cells. The prominent band at ~8 kDa corresponds to monoubiquitin.](http://www.antibodiesinc.com/cdn/shop/files/r-1405-50-ihc-wb_1600x.jpg?v=1759274456)
Left: Immunofluorescent analysis of HeLa cells stained with rabbit antibody to ubiquitin (red, 1:1,000) by Immunocytochemistry. Cells were co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. [A] Control HeLa cells maintained in normal medium; [B] HeLa cells treated with 10 uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions [B]. Note the diffuse cytoplasmic ubiquitin staining in control cells [A] and well-defined ubiquitin positive inclusions in the Lc treated cells [B]. Right: Western blot analysis of HEK293 cell lysates using rabbit antibody to ubiquitin (green, 1:5,000). [1] protein standard, [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10 um for 16 hours. The blot was simultaneously probed with a mouse antibody to beta-tubulin as loading control (red, lanes 2 and 3). The smear detected above the 200 kDa standard represents accumulations of ubiquitinated proteins in the Lc-treated cells. The prominent band at ~8 kDa corresponds to monoubiquitin.
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