Anti-Saporin Antibody

Name: Anti-Saporin Antibody
Brand: Biosensis
SKU: R-025-100
Price: 327.0 USD
Availability: InStock

Our Anti-Saporin rabbit polyclonal primary antibody detects plant Saporin, and is whole serum. It is validated for use in ELISA, IHC-Frozen, WB.

Western blot analysis using rabbit polyclonal anti-saporin antisera (R-025-100). HRP-labelled sheep anti-rabbit was used as the secondary antibody and the immunocomplex was then visualised by DAB. The expected molecular weight of the purified saporin is 29.5 kDa. SC: Spinal Cord homogenate (rat).

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Species Reactivity

Plant

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-025-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Saporin

Scientific Background

Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC, WB

Host Species

Rabbit

Immunogen

Saporin, whole molecule

Immunogen Species

Plant

Species Reactivity

Plant

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:200-1:2000
IHC: 1:200-1:2000
ICC: 1:200-1:2000
ELISA: 1:200-1:2000

Conjugate

Unconjugated

Specificity

Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Saponaria officinalis; Common soapwort

UniProt ID

P20656

Shipping

25°C (ambient)

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Anti-Saporin Antibody

SKU: R-025-100

Product Details

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