Our Arl13b antibody from NeuroMab is KO validated and works great in IHC, ICC, & WB!
Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat # 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.
Highly enriched on the ciliary membrane, Arl13b plays an important role in cilia architecture, protein trafficking and Sonic hedgehog signaling.
ADP-ribosylation factor-like protein 13b (Arl13b, also known as ADP-ribosylation factor-like protein 2-like 1) is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Aside from cilia, Arl13b is also expressed in neuroepithelial cells and developing radial glia of the developing cerebral cortex.
Arl13b is ancient, predicted to be present in the last common eukaryotic ancestor. While Arl13b’s role in regulating cilia length is from within cilia, Arl13b regulates Sonic hedgehog (Shh) signaling from outside the primary cilia. Missense mutations in the ARL13B gene cause the ciliopathy Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation.
Western blot of our Arl13b antibody shows specific labeling of Arl13b in Arl13b knockout (KO) mouse embryonic fibroblasts (MEF).