BCIP/NBT 1-Component AP Membrane Substrate (SUBB)
BCIP/NBT 1-Component AP Membrane Substrate (SUBB) is a ready-to-use formulation containing BCIP(5-bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) in a mildly alkaline buffer. SUBB is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUBB reacts with AP yielding a dark blue reaction product.
BCIP/NBT 1-Component AP Membrane Substrate (SUBB)
BCIP/NBT 1-Component AP Membrane Substrate (SUBB) is a ready-to-use formulation containing BCIP(5-bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) in a mildly alkaline buffer.
SUBB is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUBB reacts with AP yielding a dark blue reaction product.
Bulk volumes are available upon request.
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
9.6-10
1X
United States
3 years from date of manufacture
- Allow product to equilibrate to room temperature (25°C) prior to use.
- Completely cover the membrane surface with the substrate. The substrate will react with phosphatase creating a purple-blue reaction product.
- To stop the reaction, rinse the developed membrane with distilled water.
- For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.