Our Anti-Kv2.2 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N372B/60. It is KO validated, detects mouse and rat Kv2.2 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/60 (red) and Hoechst nuclear stain (blue.
Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N372B/60
IgG2b
ICC, IHC, WB
Mouse
Kcnb2
120 kDa
Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (accession number Q63099) produced recombinantly in E. Coli
Mouse, Rat
AB_2315868
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Does not cross-react with Kv2.2 short isoformDoes not cross-react with Kv2.1
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)
Irie, T., et al. 2021. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. eNeuro, ENEURO.0515-20.2021.
Bishop, H.I., et al. 2018. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Frontiers in Molecular Neuroscience, 1.
Speca, D.J., et al. 2014. Deletion of the Kv2.1 delayed rectifier potassium channel leads to neuronal and behavioral hyperexcitability.. Genes, Brain and Behavior, 394-408.
Kirmiz, M., et al. 2018. Remodeling neuronal ER-PM junctions is a conserved nonconducting function of Kv2 plasma membrane ion channels. MolecularBiology of the Cell, 2410-2432.
Bishop, H.I., et al. 2015. Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons.. J Neurosci, 14922-14942.
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