Anti-Kv2.1 Potassium Channel Subunit Antibody (L80/21)

Name: Anti-Kv2.1 Potassium Channel Subunit Antibody
Brand: NeuroMab
SKU: 75-315
Price: 329.0 USD
Availability: InStock

5 Citations

Our Anti-Kv2.1 potassium channel subunit mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L80/21. It is KO validated, detects human, mouse, and rat Kv2.1 potassium channel subunit, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude brain membranes from adult rat (RBM) and Kv2.1 wild-type (WT) or knockout (KO) mouse probed with L80/21 (left) or N52A/42 (right) TC supe.

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-315

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv2.1 potassium channel subunit

Scientific Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L80/21

Isotype

IgG3

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnb1

Molecular Weight

105-125 kDa (varies with cell background due to phosphorylation)

Antigen

Synthetic peptide amino acids 837-853 (HMLPGGGAHGSTRDQSI, cytoplasmic Cterminus) of rat Kv2.1 (accession number P15387)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315863

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily B member 1 (Delayed rectifier potassium channel 1) (DRK1) (Voltage-gated potassium channel subunit Kv2.1)

UniProt ID

P15387

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34750263	1:100	Vierra, N.C., et al. 2021. Regulation of neuronal excitation-transcription coupling by Kv2.1-induced clustering of somatic L-type Ca2+ channels at ER-PM junctions. PNAS: USA, .

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33837049	1ug/ml	Irie, T., et al. 2021. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. eNeuro, ENEURO.0515-20.2021.
33132203	not listed	Hawkins, N.A., et al. 2021. Epilepsy and neurobehavioral abnormalities in mice with a dominant-negative KCNB1 pathogenic variant. Neurobiology Disorders, 105141.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31015414	1:500	Raus Balind, S., et al. 2019. Diverse synaptic and dendritic mechanisms of complex spike burst generation in hippocampal CA3 pyramidal cells. Nature Communications, 1859.

Western Blot: Rat
PMID	Dilution	Publication
31639389	1:100	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. Journal of molecular and cell cardiology, 93–106.

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Anti-Kv2.1 Potassium Channel Subunit Antibody (L80/21)

SKU: 75-315

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kv2.1 Potassium Channel Subunit Antibody

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