Products: Primary Antibodies
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
Brand |
Catalog # GFAP |
Volume 200 µL |
Concentration 2 mg/mL |
Price $245.00 |
Validation Images
Product Details
Antibody Type:
Polyclonal
Host Species:
Chicken
Species Reactivity:
Human Mouse Rat
Applications:
WB
Western Blot
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
AT
Array Tomography
EM
Electron Microscopy
CHIP
Chromatin Immunoprecipitation
DB
Dot Blot
ICC IHC WB
Target Description:
Human Glial Fibrillary Acidic Protein (GFAP) is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in humans result in Alexander’s disease, one of the leukodystrophies.
Immunogen:
Chickens were immunized with recombinant GFAP (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.
Protein Name(s):
Glial fibrillary acidic protein (GFAP)
Gene Name(s):
GFAP
Antibody Registry ID:
AB_2313547
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
Molecular Weight:
50 kDa
Western Dilution Range:
1:1000-1:2000
IHC Dilution Range:
1:500-1:1000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
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