Apo 3/7 HTS Assay Kit

A homogenous fluorescent assay that utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The assay can be used for high throughput screening.



SKU: APO200-2

Size: 100 Tests
Price:
Sale price$310

Apoptosis is an evolutionarily conserved form of cell suicide, which follows a specialized cellular process. The central component of this process is a cascade of proteolytic enzymes called caspases. These enzymes participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in cleavage of protein substrates, causing the disassembly of the cell. Caspases have been identified in organisms ranging from C. elegans to humans. The mammalian caspases play distinct roles in apoptosis and inflammation. In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). An active caspase consists of two large (~20 kD) and two small (~10 kD) subunits to form two heterodimers which associate in a tetramer. As is common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity.

Caspase 3, also known as CPP-32, Apopain or Yama, is a key effector caspase in the apoptotic pathway. It is present in many different cell lineages and is responsible for the cleavage of a variety of molecules such as poly ADP-ribose polymerase (PARP), protein kinase C, actin a DNA-dependent protein kinase.

This kit utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase 3/7, from the quenching DEVD sequence, the free dye excites at 488 nm and emits at 515-530 nm. The Apo 3/7 HTS Assay Kit provides a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.

Caspase-3/7
Fluorescence Plate Reader
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APO200-2: 100 Tests
  • Part# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 1 Vial (-20C)
  • Part# 3005: Cell Lysis Buffer, 1 Bottle (2-8C)

  • APO200-3: 500 Tests
  • Part# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 5 Vials (-20C)
  • Part# 3005: Cell Lysis Buffer, 5 Bottles (2-8C)

  • APO200-4: 1000 Tests
  • Part# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 10 Vials (-20C)
  • Part# 3005: Cell Lysis Buffer, 10 Bottles (2-8C)
  • Product Specific References

    PMID Publication
    34024231Bailus, B. J., et al. 2021. Modulating FKBP5/FKBP51 and autophagy lowers HTT (huntingtin) levels. Autophagy, 4119-4140.
    24960609Tourette, C., et al. 2014. The Wnt receptor Ryk reduces neuronal and cell survival capacity by repressing FOXO activity during the early phases of mutant huntingtin pathogenicity. PLoS Biol, e1001895.
    24407293Tourette, C., et al. 2014. A Large Scale Huntingtin Protein Interaction Network Implicates Rho GTPase Signaling Pathways in Huntington Disease. J Biol Chem, 6709-6726.
    26232724Kim, H. J., et al. 2015. Intracellular transduction of TAT-Hsp27 fusion protein enhancing cell survival and regeneration capacity of cardiac stem cells in acute myocardial infarction. J Control Release, 55-72.
    24333653Castri, P., et al. 2015. Poly(ADP-ribose) polymerase-1 and its cleavage products differentially modulate cellular protection through NF-kB-dependent signaling. Biochim Biophys Acta, 640-51.
    22709585Siddiqui, A., et al. 2012. Mitochondrial DNA damage Is associated with reduced mitochondrial bioenergetics in Huntington's disease. Free Radic Biol Med, 1478-88.

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