Name: Anti-Neu-N (Fox 3) Antibody
Brand: Aves Labs
SKU: NUN
Price: 425.0 USD
Availability: InStock

Antipeptide antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001020102) and human (NP_001076044) gene products. These antibodies have been validated with human, mouse and rat tissues.

Clonality

Polyclonal

Applications

ICC, IHC

Host Species

Chicken

Gene Name

RBFOX3

Molecular Weight

46, 48 kDa

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Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult mouse hippocampal formation stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution). NOTE: "HippocampusCA1.2.1B.jpg" is a blow up of the central section of this photomicrograph.

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult Organ of Corti stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, 1:1000 dilution).

Immunohistochemical photomicrograph of adult dentate gyrus of the hippocampal formation. (Upper left) NeuN stains all of the granule cells (1:1000 dilution). BrdU (bromodeoxyuridine) stains proliferating cells. Note that some granules cells of the dentate gyrus were labeled with BrdU, indicating that they were in the G phase of their cell cycle when BrdU was available. Felix Eckenstein, University of Vermont.

Immunohistochemical photomicrograph of neurons in cerebral cortex from a patient with Alzheimer's Disease. Parafin-embedded section. Anti-NeuN (Aves Labs, 1:1000 dilution); 2˚ antibody was H-1004 (HRP-labeled goat anti-chicken IgY, Aves Labs, 1:1000 dilution).

Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Volume: 1000 µL

Variant

Price:

$425

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Product Details

Target

Neu-N (Fox 3)

Target Description

Human Neu-N (Fox 3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and mouse antibodies against various cytoplasmic neuronal antigens.

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

RBFOX3

Molecular Weight

46, 48 kDa

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313556

Storage

Store at 4˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

Dilution Ranges

IHC: 1:1000-1:2000

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)

UniProt Details

UniProt (Immunogen Species): A6NFN3

Citations (7)

Malm T, Mariani M, Donovan LJ, Neilson L, Landreth GE (2014), 'Activation of the nuclear receptor PPARd is neuroprotective in a transgenic mouse model of Alzheimer’s disease through inhibition of inflammation.' Journal of Neuroinflammation. 10.1186/s12974-014-0229-9.
Malik A, Kondratov RV, Jamasbi RJ, Geusz ME (2015), 'Circadian Clock Genes Are Essential for Normal Adult Neurogenesis, Differentiation, and Fate Determination.' Plos One. 10.1371/journal.pone.0139655.
Huh CJ, Zhang B, Victor MB, Dahiya S, Batista LF, Horvath S, Yoo AS (2016), 'Maintenance of age in human neurons generated by microRNA-based neuronal conversion of fibroblasts.' eLife. 10.7554/eLife.18648.
Abernathy DG, Kim WK, McCoy MJ, Lake AM, Ouwenga R, Lee SW, Xing X, Li D, Lee HJ, Heuckeroth RO, Dougherty JD, Wang T, Yoo AS (2017), 'MicroRNAs Induce a Permissive Chromatin Environment that Enables Neuronal Subtype- Specific Reprogramming of Adult Human Fibroblasts.' Cell Stem Cell. 10.1016/j.stem.2017.08.002.
Butler RK, Ehling S, Barbar M, Thomas J, Hughes MA, Smith CE, Pogorelov VM, Aryal DK, Wetsel WC, Lascelles BDX (2017), 'Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia.' Neuroscience Letters. 10.1016/j.neulet.2017.09.025.
Victor MB, Richner M, Olsen HE, Lee SW, Monteys AM, Ma C, Huh CJ, Zhang B, Davidson BL, Yang XW, Yoo AS (2018), 'Striatal neurons directly converted from Huntington’s disease patient fibroblasts recapitulate age-associated disease phenotypes.' Nature Neuroscience. 10.1038/s41593-018-0075-7.
Michele J. Alves, Mustafa Goksel, Behiye Kaya, Hasnaa Mostafa, Patrick Gygli, Julie Stephens,y Summer Fair, Jose´ J. Otero, and Catherine M. Czeisler (2019), 'CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus. The American .' Journal of Pathology. 10.1016/j.ajpath.2018.10.020.

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Anti-Neu-N (Fox 3) Antibody

Antipeptide antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001020102) and human (NP_001076044) gene products. These antibodies have been validated with human, mouse and rat tissues.