Antipeptide antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001020102) and human (NP_001076044) gene products. These antibodies have been validated with human, mouse and rat tissues.
Human, Mouse, Rat
46, 48 kDa
Neu-N (Fox 3)
Human Neu-N (Fox 3) is a 33,873 dalton (312 amino acid) RNA-binding protein. This protein is found in the nuclei of virtually all post-mitotic central and peripheral neurons and has been used extensively as a general neuronal marker. In these studies, Neu-N (Fox 3) stains neuronal nuclei relatively uniformly, except in the region of the nucleolus, providing a distinctive donut (i.e., torus) shape. Since this antigen is not seen in the cytoplasm, it offers the advantage of being useful in co-immunostaining studies with rabbit and mouse antibodies against various cytoplasmic neuronal antigens.
46, 48 kDa
Human, Mouse, Rat
Store at 4˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
12 months from receipt of product
RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)
UniProt (Immunogen Species): A6NFN3
Product Specific References for Applications and Species
|29403030||1:500||Victor, M.B., et al. 2018. Striatal neurons directly converted from Huntington''''s disease patient fibroblasts recapitulate age-associated disease phenotypes. Nature Neuroscience, 341-352.|
|28886366||not listed||Abernathy, D.G., et al. 2017. MicroRNAs Induce a Permissive Chromatin Environment that Enables Neuronal Subtype- Specific Reprogramming of Adult Human Fibroblasts. Cell Stem Cell, 332-348.|
|27644593||1:500||Huh, C.J., et al. 2016. Maintenance of age in human neurons generated by microRNA-based neuronal conversion of fibroblasts. eLife, e18648.|
|36510342||not listed||Davis, D., et al. 2022. Localisation of Oestrogen Receptors in Stem Cells and in Stem Cell-Derived Neurons of the Mouse. Journal of Neuroendocrinology, e13220.|
|26439128||1:1000||Malik, A., et al. 2015. Circadian Clock Genes Are Essential for Normal Adult Neurogenesis, Differentiation, and Fate Determination. Plos One, 0.|
|34142098||1:200||Li, X., et al. 2021. Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve. STAR Protocols, 100555.|
|31059735||1:1000||Beligala, D.H., et al. 2019. Musashi-2 and related stem cell proteins in the mouse suprachiasmatic nucleus and their potential role in circadian rhythms. International Journal of Developmental Neuroscience, 44-58.|
|30579783||1:400||Alves, M.J., et al. 2019. CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus.. Journal of Pathology, 426-439.|
|28916300||1:500||Butler, R.K., et al. 2017. Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia. Neuroscience Letters, .|
|25592770||not listed||Malm, T., et al. 2014. Activation of the nuclear receptor PPARδ is neuroprotective in a transgenic mouse model of Alzheimer''''s disease through inhibition of inflammation. Journal of Neuroinflammation, 7.|