Anti-QKI-7 Antibody (N183/15)

Name: Anti-QKI-7 Antibody
Brand: NeuroMab
SKU: 75-200
Price: 329.0 USD
Availability: InStock

Our Anti-QKI-7 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N183/15. It detects human and mouse QKI-7, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b and untagged Kv2.1 plasmids and probed with N183/15 (left) and mouse anti-Flag (right.

Western blot of protein extracted from UD hESCs, DE cells, and CM cells probed with antibodies for QKI5 (magenta, top panel) and panQKI (green), QKI6 (magenta, middle panel) and panQKI (green), or QKI7 (magenta, bottom panel; ND denotes not detectable) and panQKI (green). Image from publication CC-BY-4.0. PMID: 35544276

Double immunofluorescence revealing pruned Lewy Neurites (pSyn, Red) and the cytoplasms of mature mouse OLs (QKI-7, cat. 75-200, 1:500; green) at the level of the anterior commissure of a mouse 6 months after the intrastriatal injection of α-Syn PFFs. Horizontal brain section. Nuclei are counterstained with DRAQ7 (white). Image from publication CC-BY-4.0. PMID:36830639

Adult rat hippocampus immunohistochemistry.

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Species Reactivity

Human, Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-200

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

QKI-7

Scientific Background

QKI7 is a member of the Signal Transduction and Activation of RNA proteins (STAR proteins) family. QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. QKI7 is a RNA-binding protein. QKI6 has roles in the regulation of splicing, export and stability of mRNA. It is involved in myelinization. It is a cytoplasmic isoform. Diseases associated with this gene include Angiocentric Glioma, 6Q Terminal Deletion Syndrome and Schizophrenia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N183/15

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Qki Qk QKI HKQ Qk1 Qka1

Molecular Weight

36-38 kDa

Antigen

Synthetic peptide amino acids 303-317 (LEWIEMPVMPDISAH, C-terminal tail) of human QKI-7 (accession number Q96PU8-5)

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2173148

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

Does not cross-react with QKI-5, QKI-6 or QKI-7b

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Protein quaking (Hqk) (HqkI)

UniProt ID

Q96PU8-5

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32732889	1:100	Yang, C., et al. 2020. Targeting QKI-7 in vivo restores endothelial cell function in diabetes. Nature Communications, 3812.

Immunohistochemistry: Human
PMID	Dilution	Publication
27029405	10ug/ml	de Bruin, R.G., et al. 2014. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.. Nature Communications, 10846.
23963726	not listed	van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36830639	1:500	De Nuccio, F., et al. 2023. Oligodendrocytes Prune Axons Containing α-Synuclein Aggregates In Vivo: Lewy Neurites as Precursors of Glial Cytoplasmic Inclusions in Multiple System Atrophy?. Biomolecules, 269.
27454326	1:100	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.
27196066	not listed	Suiko, T., et al. 2016. Expression of Quaking RNA-Binding Protein in the Adult and Developing Mouse Retina. PLoS One, e0156033.

Western Blot: Human
PMID	Dilution	Publication
37379838	not listed	Zhao, Z, et al. 2023. QKI Shuttles Internal m7G-modified Transcripts into Stress Granules and Modulates mRNA Metabolism. Cell, .
35544276	not listed	Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334.
24449917	1:1000	Chan, C.B., et al. 2014. PIKE is essential for oligodendroglia development and CNS myelination.. PNAS: USA, 1993-1998.

Western Blot: Mouse
PMID	Dilution	Publication
32732889	1:1000	Yang, C., et al. 2020. Targeting QKI-7 in vivo restores endothelial cell function in diabetes. Nature Communications, 3812.
27454326	1:1000	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.
27196066	not listed	Suiko, T., et al. 2016. Expression of Quaking RNA-Binding Protein in the Adult and Developing Mouse Retina. PLoS One, e0156033.

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Anti-QKI-7 Antibody (N183/15)

SKU: 75-200

Product Details

Product Specific References for Applications and Species

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