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Products: Primary Antibodies

Anti-Integrin alpha-M (CD11b, ΜΑC-1, OX-42 Antigen) Antibody

Aves Labs Logo from Aves Labs

 Datasheet

Product Summary:

Our anti-Integrin Alpha-M (MAC-1) antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001076429.1) and human gene products (NP_001139280.1). These antibodies have been validated with human, mouse and rat tissues.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
MAC
Volume
1000 µL
Concentration
100 µg/mL
Price
$399.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC WB

Target Description:

Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 mouse monoclonal antibody.

Immunogen:

Hens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.

Protein Name(s):

Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)

Gene Name(s):

ITGAM CD11B CR3A

Antibody Registry ID:

AB_2313548


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

170 kDa

Western Dilution Range:

1:2000-1:5000

IHC Dilution Range:

1:2000-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.


Citations and References