Products: Primary Antibodies

Anti-Integrin alpha-M (CD11b, ΜΑC-1, OX-42 Antigen)

PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # MAC		Volume Regular vials contain 1000 ul of this antibody; Sampler vials contain 200 ul of this antibody.	Concentration 100 µg/ml	Price $399.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:

IHC WB

Immunogen:

Hens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.

Protein Name(s):

Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)

Gene Name(s):

ITGAM CD11B CR3A

Antibody Registry ID:

Default Title: AB_2313548

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Expected Banding Pattern:

170 kDa

Western Dilution Range:

1:2000-1:5000

IHC Dilution Range:

1:2000-1:5000

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.

Citations and References

Merlo S, Spampinato SF, Beneventano M, Sortino MA. (2018), 'The contribution of microglia to early synaptic compensatory responses that precede β-amyloid-induced neuronal death.' Nature. 10.1038/s41598-018-25453-1.