Products: Primary Antibodies
Anti-Integrin alpha-M (CD11b, ΜΑC-1, OX-42 Antigen) Antibody
from Aves Labs
Our anti-Integrin Alpha-M (MAC-1) antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001076429.1) and human gene products (NP_001139280.1). These antibodies have been validated with human, mouse and rat tissues.
PRODUCT OPTIONS
| Brand | Catalog # | Option | Volume | Concentration | Price | |
|---|---|---|---|---|---|---|
| Brand |
Catalog # MAC |
Volume 1000 µL |
Concentration 100 µg/mL |
Price $399.00 |
Validation Images
Product Details
Polyclonal
Chicken
Human Mouse Rat
IHC WB
Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 mouse monoclonal antibody.
Hens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.
Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)
ITGAM CD11B CR3A
AB_2313548
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
170 kDa
1:2000-1:5000
1:2000-1:5000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Antibodies were analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.
Citations and References
- Merlo S, Spampinato SF, Beneventano M, Sortino MA. (2018), 'The contribution of microglia to early synaptic compensatory responses that precede β-amyloid-induced neuronal death.' Nature. 10.1038/s41598-018-25453-1.