Products: Primary Antibodies

Anti-Green Fluorescent Protein (GFP) Antibody


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PRODUCT OPTIONS

Catalog # Option Concentration Price*
Catalog #
GFP-1010
Option
100 µL
Concentration
10.0 mg/ml
Price
$199.00
Catalog #
GFP-1020
Option
400 µL
Concentration
10.0 mg/ml
Price
$419.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ELISA ICC IHC WB

Immunogen:

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with thimerosal, and then filter-sterilized.

Antibody Registry ID:

100 µL: AB_2307313
400 µL: AB_10000240


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Dilution Range:

1:5000-1:10000

IHC Dilution Range:

1:2000-1:5000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.