Name: Anti-CREB (Ser133) Antibody
Brand: PhosphoSolutions
SKU: p1010-133
Price: 415.0 USD
Availability: InStock

Our Anti-CREB (Ser133) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Sheep, Xenopus, Zebrafish

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

CREB1

Molecular Weight

45 kDa

Go to Full Product Details

Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100). These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).

Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).

Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-CREB (Ser133) Antibody

Product Details

Target

CREB (Ser133)

Target Description

It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser-133), and phosphorylation of Ser-133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

CREB1

Molecular Weight

45 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Sheep, Xenopus, Zebrafish

Antibody Registry ID

AB_2492066

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser133

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Active transcription factor CREB antibody, cAMP response element binding protein 1 antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, cAMP-responsive element-binding protein 1 antibody, CREB antibody, CREB-1 antibody, CREB1 antibody, CREB1_HUMAN antibody, Cyclic AMP-responsive element-binding protein 1 antibody, MGC9284 antibody, OTTHUMP00000163864 antibody, OTTHUMP00000163865 antibody, OTTHUMP00000206660 antibody, OTTHUMP00000206662 antibody, , TTHUMP00000206667 antibody, Transactivator protein antibody

UniProt Details

UniProt (Human): P16220
UniProt (Immunogen Species): P15337

NCBI Gene ID

81646

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blotting: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
27766068	not listed	Bu, W., et al. 2016. Mild traumatic brain injury produces neuron loss that can be rescued by modulating microglial activation using a CB2 receptor inverse agonist. Frontiers in neuroscience, 10, p.449.

Western Blotting: Mouse
PMID	Dilution	Publication
31216095	1:500	Abdurakhmanova, S., et al. 2019. . Abnormal behavior, striatal dopamine turnover and opioid peptide gene expression in histamine‐deficient mice. Genes, Brain and Behavior, p.e12595.
29367010	not listed	Yau, S.Y., et al. 2018. Chronic minocycline treatment improves hippocampal neuronal structure, NMDA receptor function, and memory processing in Fmr1 knockout mice. Neurobiology of disease, 113, pp.11-22.
28650315	1:300	Du, J., et al. 2016. Transient acidosis while retrieving a fear-related memory enhances its lability. eLife, 6, p.e22564.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-CREB (Ser133) Antibody

Our Anti-CREB (Ser133) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.