Anti-CREB (Ser133) Antibody

4 Citations

Our Anti-CREB (Ser133) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100). These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).
Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100). These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).

Mouse, Rat
IHC, WB
Rabbit

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Data Sheet
Trial Size Trial Size Pooled Serum Pooled Serum

SKU: p1010-133

Volume: 100 µL
Price:
$430.00

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse
  • Western Blotting: Mouse
Immunohistochemistry: Mouse
PMID Dilution Publication
27766068 not listed Bu, W., et al. 2016. Mild traumatic brain injury produces neuron loss that can be rescued by modulating microglial activation using a CB2 receptor inverse agonist. Frontiers in neuroscience, 10, p.449.
Western Blotting: Mouse
PMID Dilution Publication
31216095 1:500 Abdurakhmanova, S., et al. 2019. . Abnormal behavior, striatal dopamine turnover and opioid peptide gene expression in histamine‐deficient mice. Genes, Brain and Behavior, p.e12595.
29367010 not listed Yau, S.Y., et al. 2018. Chronic minocycline treatment improves hippocampal neuronal structure, NMDA receptor function, and memory processing in Fmr1 knockout mice. Neurobiology of disease, 113, pp.11-22.
28650315 1:300 Du, J., et al. 2016. Transient acidosis while retrieving a fear-related memory enhances its lability. eLife, 6, p.e22564.

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