Antibody Purification

Methodology: Affinity Purification

• Typically used for purification of polyclonal antibodies.
• Includes custom column preparation & affinity purification (25mL) for the 1st run.
• Antibody at >90% purity in your buffer of choice.

Methodology: Protein A/G Purification
(Maxi-scale: up to 100mg)

• Allows purification of total IgG from a sample.
• Commonly used for purification of both monoclonal and polyclonal antibodies.
• Antibody at >90% purity in your buffer of choice.

Methodology: Protein A/G Purification
(Midi-scale: 1-5mg)

• Allows purification of total IgG from a sample.
• Commonly used for purification of both monoclonal and polyclonal antibodies.
• Antibody at >90% purity in your buffer of choice.

Methodology: Protein A/G Purification
(Mini-scale: 100-400ug)

• Allows purification of total IgG from a sample.
• Commonly used for purification of both monoclonal and polyclonal antibodies.
• Antibody at >70% purity in your buffer of choice.

Methodology: Adsorption (Removal of Cross-Reactive Species)

• Serum, or other antibody-containing matrix, is applied to a sequence of columns to remove various cross-reactive antibodies.
• Antibody at >90% purity in your buffer of choice.