Our Anti-NDRG2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NDRG2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.
COS-7 cells expressing NDRG2-FLAG were subjected to immunofluorescent staining using Aves Labs chicken anti-NDRG2 antibody (visualized in green) and rabbit anti-FLAG antibody (visualized in red). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. The staining revealed a complete overlap between the signal from the chicken anti-NDRG2 antibody and the anti-FLAG antibody specifically in transfected cells.
NDRG2, or N-myc downstream-regulated gene 2, is a downstream target of the N-myc proto-oncogene. NDRG2 was initially identified for its role in cell differentiation and response to cellular stress. Multiple studies have determined that NDRG2 mRNA is downregulated or undetectable in several human cancers, and over-expression of NDRG2 inhibited the proliferation of cancer cells. Commonly regarded as a tumor suppressor, NDRG2 also plays a crucial role in regulating cell proliferation, apoptosis, and metastasis. In neuroscience, studies of astrocytes in the central nervous system have implicated NDRG2 in the pathogenesis of many neurological diseases not limited to tumors; including stroke, neurodegenerative diseases, and psychiatric disorders. Its involvement in maintaining the integrity of the blood-brain barrier adds to its significance in neurobiology (Takarada-Iemata et al. Glia, 2018). Drug therapies to modulate the expression of NDGR2 may be able to help patients suffering from disease states ranging from multiple cancer types to Alzheimer’s, Parkinson’s, and depression.
Affinity-Purified IgY
200 µg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
NDRG2
41 kDa
Recombinant protein encompassing the N-terminal ~150 amino acids of human NDRG2
Human, Mouse, Rat
AB_3094925
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from receipt of product
Ice packs
SYLD, KIAA1248, N-Myc Downstream-Regulated Gene 2 Protein, N-Myc Downstream Regulator 2, NDR1-Related Protein NDR2, Cytoplasmic Protein Ndr1, Syld709613 Protein
