Products: Primary Antibodies

Anti-Vimentin protein


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
VIM		 Volume
Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.		 Concentration
300 µg/ml 		Price
$399.00

Validation Images

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Protein Name(s):

Vimentin

Gene Name(s):

VIM

Antibody Registry ID:

AB_2313565

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

52 kDa

Western Dilution Range:

1:2000-1:5000

IHC Dilution Range:

1:1000-1:2000

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.